1. Start with the Michaelis-Menten equation and convert it to a double-reciprocal equation. Show how you would make a linear Lineweaver-Burk plot using this equation. 2. Based on th chart make a Lineweaver-Burk plot and later use the plot to complete the information in the table.

1. Start with the Michaelis-Menten equation and convert it to a double-reciprocal equation. Show how you would make a linear Lineweaver-Burk plot using this equation. 2. Based on th chart make a Lineweaver-Burk plot and later use the plot to complete the information in the table.

Essentials of Pharmacology for Health Professions

7th Edition

ISBN:9781305441620

Author:WOODROW

Publisher:WOODROW

Chapter19: Analgesics, Sedatives, And Hypnotics

Section: Chapter Questions

Problem 15CRQ

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Question

1. Start with the Michaelis-Menten equation and convert it to a double-reciprocal equation. Show how you would make a linear Lineweaver-Burk plot using this equation.

2. Based on th chart make a Lineweaver-Burk plot and later use the plot to complete the information in the table.

[S],
V, mm/sec
(no inhibitor)
mM
.5
12
2.0
20
5.0
29
13.5 35
45.0
40
V, mm/sec
(+inhibitor)
5.5
9
13
16
18
Reaction
No inhibitor
+ inhibitor
Type of
inhibition
Best fit line
equation
Km or
Km (app)
Vmax or
Vmax (app)

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Step 1: Michaelis Menten equation and Lineweaver Burk Plot

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Step 2: Lineweaver Burk Plot for the given enzyme

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Step 3: Calculation for Km, vmax, Km' and vmax'

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Step 4: Type of inhibition

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