1. We have 2 liters of a 3 M solution of Tris-HCl. We need to make 500 mL of 300 mM solution. We also have solid componds of Tris-HCl. The molecular weight of Tris-HC1 is 157.60 a) What is the starting concentration (C1)? b) What is the final concentration (C2)? c) What is the starting volume (V1)? d) What is the final volume (V2)? e) What information presented above is not necessary for your calculation? HO H₂N OH.HCI -OH f) Write out the equation used to solve this. Show all your work. Are we able to make a solution using this dilution method using our starting materials? If not, propose another way to make the desired concentration and volume of the solution. Show your work.

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1. We have 2 liters of a 3 M solution of Tris-HCl. We need to make 500 mL of 300 mM solution. We also
have solid componds of Tris-HCl. The molecular weight of Tris-HC1 is 157.60
a) What is the starting concentration (C1)?
b) What is the final concentration (C2)?
c) What is the starting volume (V1)?
d) What is the final volume (V2)?
e) What information presented above is not necessary for your calculation?
HO
H₂N
OH HCI
-OH
f) Write out the equation used to solve this. Show all your work. Are we able to make a solution
using this dilution method using our starting materials? If not, propose another way to make the
desired concentration and volume of the solution. Show your work
Transcribed Image Text:1. We have 2 liters of a 3 M solution of Tris-HCl. We need to make 500 mL of 300 mM solution. We also have solid componds of Tris-HCl. The molecular weight of Tris-HC1 is 157.60 a) What is the starting concentration (C1)? b) What is the final concentration (C2)? c) What is the starting volume (V1)? d) What is the final volume (V2)? e) What information presented above is not necessary for your calculation? HO H₂N OH HCI -OH f) Write out the equation used to solve this. Show all your work. Are we able to make a solution using this dilution method using our starting materials? If not, propose another way to make the desired concentration and volume of the solution. Show your work
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