Question1 ustifySW2Xj1. What is the superhelical density (o) of a closed-circular DNA with a length of 4,200 bp and a linkingnumber (Lk) of 374? What is the superhelical density (a) of the same DNA when Lk = 412? In eachcase, is the DNA negatively or positively supercoiled?4all answers!2. The complete genome of the simplest bacterium known, Mycoplasma genitalium, is a circular DNAmolecule with 580,070 bp. Calculate the molecular weight (assume the molecular weight of anucleotide pair is 650 g/mol) and contour length (when relaxed) of this molecule. What is Lko for thisMycoplasma chromosome?3. The T4-like bacteriophage JS98 has a DNA of molecular weight 1.11 x 108 grams/mole (g/mol)contained in a head about 100 nanometers (nm) long.(a) Calculate the length of the DNA molecule (assume the molecular weight of a nucleotide pair is 650g/mol) and compare it with the length of the JS98 head.(b) Consult the online database Genome (https://www.ncbi.nlm.nih.gov/data-hub/genome/). What isthe exact number of base pairs in the JS98 genome? Provide a citation (make sure it follows theexact format as described in the Biology Style Manual).4. A closed-circular DNA molecule in its relaxed form has an Lk of 500. Approximately how many basepairs are in this DNA? How does the Lk change (increases, decreases, does not change, becomesundefined) in each of the following situations?w $3(a) A protein complex binds, wrapping the DNA round it to form a solenoidal supercoil.(b) One DNA strand is broken.ED#(c) DNA gyrase and ATP are added to the DNA in solution.(d) The double helix is denatured by heat.BC$4RFF4V%5FSTGB^6FGYH&7UNPrtScnJ*8Home1M(9KEndOVOLPgUpP-APgDnAlt¡

"Since you have asked multiple questions, we will solve the first question for you. If you want a…

1. What is the superhelical density (o) of a closed-circular DNA with a length of 4,200 bp and a linking number (Lk) of 374? What is the superhelical density (o) of the same DNA when Lk = 412? In each case, is the DNA negatively or positively supercoiled?

1. What is the superhelical density (o) of a closed-circular DNA with a length of 4,200 bp and a linking number (Lk) of 374? What is the superhelical density (o) of the same DNA when Lk = 412? In each case, is the DNA negatively or positively supercoiled?

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 8P: Human Genome Replication Rate Assume DNA replication proceeds at a rate of 100 base pairs per second...

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Question

Question1

ustify
S
W
2
X
j
1. What is the superhelical density (o) of a closed-circular DNA with a length of 4,200 bp and a linking
number (Lk) of 374? What is the superhelical density (a) of the same DNA when Lk = 412? In each
case, is the DNA negatively or positively supercoiled?
4
all answers!
2. The complete genome of the simplest bacterium known, Mycoplasma genitalium, is a circular DNA
molecule with 580,070 bp. Calculate the molecular weight (assume the molecular weight of a
nucleotide pair is 650 g/mol) and contour length (when relaxed) of this molecule. What is Lko for this
Mycoplasma chromosome?
3. The T4-like bacteriophage JS98 has a DNA of molecular weight 1.11 x 108 grams/mole (g/mol)
contained in a head about 100 nanometers (nm) long.
(a) Calculate the length of the DNA molecule (assume the molecular weight of a nucleotide pair is 650
g/mol) and compare it with the length of the JS98 head.
(b) Consult the online database Genome (https://www.ncbi.nlm.nih.gov/data-hub/genome/). What is
the exact number of base pairs in the JS98 genome? Provide a citation (make sure it follows the
exact format as described in the Biology Style Manual).
4. A closed-circular DNA molecule in its relaxed form has an Lk of 500. Approximately how many base
pairs are in this DNA? How does the Lk change (increases, decreases, does not change, becomes
undefined) in each of the following situations?
w $
3
(a) A protein complex binds, wrapping the DNA round it to form a solenoidal supercoil.
(b) One DNA strand is broken.
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D
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(c) DNA gyrase and ATP are added to the DNA in solution.
(d) The double helix is denatured by heat.
B
C
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