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- True or false 1. Heterogenous antibodies used for structural studies can be obtained by monoclonal hybridoma technique. 2. PrP(Sc) is largely alpha-helical structure.Define the principle immunoturbidimetric C3 assay(66)A laboratory animal is scheduled to be immunized by receiving weekly injections of antigen X. Analysis of serum drawn 2 days after the last immunization would show antibodies to antigen X to be primarily of which of the following classes? (A) IgA (B) IgD (C) IgE (D)IgG (E)IgM
- In relation to immunotechnology, answer the following: explain the reason behind developing different forms of Monoclonal antibodies (chimeric MABs, humanized MABs, and fully human MABs)The graph below shows the titration curve that results when 50. mL of 0.50 Mtromethamine is titrated with 0.100 MHCl.Weigh the following observation: High titers of antibodies for EBVare found in a leukemia patient; a chronically tired, ill businessman; a healthy military recruit; and an AIDS patient. Comment on theprobable significance of antibodies to EBV in human serum.
- Why is a blocking buffer needed while running the immunoblotting of the PDVF membrane? Why is skim milk powder used in these buffers?10. What are the advantages/disadvantages of using a direct imm1. True or False: Antibodies for Western blotting can be applied directly to the SDS-PAGE gel.Warm autoimmune hemolytic anemia, WAIHA, frequently have antibody specificity of: Question 6 options: A) anti-P B) anti-I C) anti-i D) anti-e
- You have 4 ml of an antigen solution, how would you prepare a 3-fold dilution series such that you will have at least 2 ml of each dilution? Please draw a picture and explain, I don't understand what it means by 3-fold. Please explain, no copy paste asapYou have the following reagents available Rat anti-Keratin 8 antibody specific for mouse Keratin 8, Rabbit polyclonal antibody against mouse keratin 5 and antifade reagent with DAPI to stain DNA. Both primary antibodies work best at 1:500 dilution. A) What additional reagents would you need to perform immunostaining of frozen sections of mouse thymus tissue for Keratin 8 that is expressed in cortical thymic epithelial cells and Keratin 5 that is expressed in mouse medullary thymic epithelial cells? B) Create a staining protocol including all tubes and appropriate controls to stain 4 slides with frozen thymic sections. You will need 200ul of antibody staining solution for each slide.2. https://doi.org/10.1186/s12868-022-00692-1 (link to research) a) In the immunohistochemistry section of the materials and methods section the authors wrote “The number of positive cells in hotspot areas in ten high power fields (HPFs) in areas of demyelination and plaques in the brain stem were counted using the image analysis software (Lecia Application Suite Version 4.12.0, Welzlar, Germany).” Why were they looking at demyelination areas for this study? b) In the effect of mitoxantrone on histopathological changes in the brain section of the results section the authors wrote “Active plaques revealed inflammatory cellular infiltrates with abundant macrophages stuffed with myelin debris, an evidence of ongoing myelin breakdown.” What does macrophages stuffed with myelin debris have to do with the study?