14 strength Pulmocare 450mL q8h followed by 100mL of water after each feed. Calculate the mixture, and determine the pump rate in ml/hr. Soluted needed Solvent needed Flow rate in mL/hr for feeding
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- 10 mL of 0.5 M CuSO₄ * 5H₂O solution from 10 M CuSO₄ * 5H₂O solutionpls compute how to prepare the solutions1. 100.0 mL 0.0500 M stock Ca2+ solutiona. Weigh appropriate amount of pure CaCO3 (FW=100.09) to the nearest 0.1 mg into a 250 mL beaker. Add about 40.0 mL distilled water. Take note of the purity of CaCO3 used.b. Carefully and slowly add concentrated HCl to dissolve the CaCO3 solids while stirring and heating the solution in a hotplate. Do this until the CaCO3 solids are completely dissolved or until no more effervescence is observed.2. 100.0 mL 0.0500 M stock Ca2+ solutiona. Weigh appropriate amount of pure CaCO3 (FW=100.09) to the nearest 0.1 mg into a 250 mL beaker. Add about 40.0 mL distilled water. Take note of the purity of CaCO3 used.b. Carefully and slowly add concentrated HCl to dissolve the CaCO3 solids while stirring and heating the solution in a hotplate. Do this until the CaCO3 solids are completely dissolved or until no more effervescence is observed.NOTE: The stirring must be vigorous enough to dissolve the solids. It is advisable…Calculate the appropriate volume (in µL) of 3X loading buffer that should be added to 24.0 µL of a sample prior to loading the sample on an agarose gel.
- A patient is prescribed 15mmol of potassium chloride injection. The ampoule contains 20mmol in 10ml. What volume of potassium chloride (ml) is required? units – mlWhy are we using this materials and centrifuging at 15000 rpm? Lipid peroxidation experiment:Materials;• 10% TCA solution• 0.67% TBA solution• Tissue sample Procedures:The tissue sample is buffered at the ratio of 1 x 4 and crushed with the help of a blender. It is centrifuged at 15 000 rpm. Then, 2.5 mL of 10% TCA is added onto 0.5 mL of tissue sample and the tubes are mixed in vortex. It is kept in boiling water for 15 minutes and immediately cooled. After centrifugation at 5000 rpm for 15 minutes, 2 mL of each supernatant is transferred to another tube. 1 mL of 0.67% TBA is added on it and mixed in vortex. After the samples are kept in boiling water for 15 minutes and cooled immediately, their absorbance at 532 nm is recorded. The amount of MDA is calculated by utilizing the highest absorbance specific absorbance values (E = 1.56 x 105cm-1M-1 ) of the MDA-TBA complex formed at 532 nm.3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mL
- 100 mL of 2.5 X NaOH solution from 50X NaOH solutionYou record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equation1. Tools that do not need to be brought when sampling is…a. thermometerb. Hygrometerc. PH meterd. DO meter 2. In the deposition method, one of the conditions that must be met is…a. The precipitate has a high solubility or solubility productb. The precipitate formed must be quite difficult to dissolvec. The precipitate formed does not have to be quite pured. Free precipitate form