17. The table below provides the Biodrop results obtained for the DNA extracted from students 1-4 during practical 1. Analyse the data and answer question a-c below. Sample ID Nucleic Acid Conc. Unit 260/280 260/230 321,2 ng/ul 25,4 ng/ul 56,5 ng/ul 150,95 ng/ul 1 1,9 1,83 1,38 1,1 2,16 1,1 0,47 2 4 1.9 N3

17. The table below provides the Biodrop results obtained for the DNA extracted from students 1-4 during practical 1. Analyse the data and answer question a-c below. Sample ID Nucleic Acid Conc. Unit 260/280 260/230 321,2 ng/ul 25,4 ng/ul 56,5 ng/ul 150,95 ng/ul 1 1,9 1,83 1,38 1,1 2,16 1,1 0,47 2 4 1.9 N3

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 8.5P

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Show the calculations for the preparation of 0.01%, 0.001%, 0.0001%, 0.00001% standard DNA solutions via serial dilution from a 0.1% stock solution. Prepare 10.0 mL of 0.10% standard DNA solution using acetate buffer pH 4.6 as solvent. Prepare four different concentrations of the 0.10% standard DNA solution from step 1 via serial dilution to obtain 0.01%, 0.001%, 0.0001%, 0.00001% standard DNA solutions. Obtain seven clean and dry test tubes. Place 1.50 mL solutions in each tube as follows: Table 2.1. Set-up for the diphenylamine assay. Test tube # Content 1 (blank) acetate buffer pH 4.6 2 0.10% standard DNA 3 0.01% standard DNA 4 0.001% standard DNA 5 0.0001% standard DNA 6 0.00001% standard DNA 7 DNA extract from Part I Add 3.50 mL diphenylamine reagent to each tube, swirl each tube to thoroughly mix the contents and heat for 10 mins in a boiling water bath. Cool immediately under tap water. Read absorbances at 595 nm.…
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