Janet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram negative bacteria. After finishing, she realizes she mixed up the safranin and alcohol steps. What is likely to be observed when she views the slide under the microscope?     All cells will appear purple, regardless of whether they are actually Gram negative or Gram positive.     All cells will appear pink, regardless of whether they are actually Gram negative or Gram positive.     Gram negative cells will be clear, Gram positive cells will be purple.     Gram positive cells will be clear, Gram negative cells will be pink.     Cells will appear clear, regardless of whether they are actually Gram negative or Gram positive.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter7: Basic Clinical Microbiology
Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing
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  1. Janet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram negative bacteria. After finishing, she realizes she mixed up the safranin and alcohol steps. What is likely to be observed when she views the slide under the microscope?

       

    All cells will appear purple, regardless of whether they are actually Gram negative or Gram positive.

       

    All cells will appear pink, regardless of whether they are actually Gram negative or Gram positive.

       

    Gram negative cells will be clear, Gram positive cells will be purple.

       

    Gram positive cells will be clear, Gram negative cells will be pink.

       

    Cells will appear clear, regardless of whether they are actually Gram negative or Gram positive.

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