(2) It will be harder to find a specimen on a slide starting with 40x objective lens compared to 4x objective lens because the 40x objective lens has a higher power then the 4x which means the 40x will provide a much closer image then the 4x which makes it a lot harder to see. on the other hand the 4x provides a further filed of view which makes the image easier to see.
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Introduction
A microscope is a scientific tool that is used to study items that are too small for the naked eye to see. Microscopy is the science of using a microscope to study small objects and structures. Unless helped by a microscope, microscopic means invisible to the naked sight.
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- 1. after going through all 4 objectives, which would give you the largest field of view? greatest depth of focus? why? 2. how do you calculate the total magnification of a specimen? show sampleThe field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?Can someone explain the concept behind a sample that lies in the UV range in a spectrophotometer? Please explain in detail and clearly. Thank you!
- Match the term with it's definition from the lab Group of answer choices Field of View [ Choose ] occular lens x objective lens the width of the circle of light the distance from the slide to the bottom of the lens clarity of an image Working Distance [ Choose ] occular lens x objective lens the width of the circle of light the distance from the slide to the bottom of the lens clarity of an image Resolution [ Choose ] occular lens x objective lens the width of the circle of light the distance from the slide to the bottom of the lens clarity of an image Total Magnification [ Choose ] occular lens x objective lens the width of the circle of light the distance from the slide to the bottom of the lens…Instruction: FILL IN THE BLANKS 1. Manipulate the ________________ knob to bring the objective about 1 to 2 mm away from the slide. Never look into the ____________ when bringing the objective close to the slide to avoid damaging or crushing the slide. A clear field of vision should be ___________ not gray. 2. In viewing objects under the microscope, it is observed that the microscope ______________ the direction of movement and _______________ the position of the image observed. 3. In shifting to higher magnification, simply rotate the _________________ to the desired objective. If the image is not clear manipulate the __________________ knob. 4. The total magnification of the specimen as seen in the microscope is 400x. This means that the ocular magnification is _______ x and the ______________ is 40x.How does the numerical aperture of an objective lens affect resolution? Question options: Numerical aperture is the optical plane visible for each objective lens Numerical aperture decreases the amount of excess light on a specimen increasing the resolution and clarity Numerical aperture is the loss of light due to changes in density between the specimen and the objective lens Numerical aperture is a measure of the amount of light an objective lens can accept producing higher resolution and allowing smaller structures to be visualized with greater clarity
- You should always position the cells or tissues in the center of view before increasing magnification, because... You know that the image will move right as you move the slide to the left. You know that the image will move up as you move the slide down. You know that the center of the field of view will be unchanged. This is the best way to avoid breaking the slide with an objectiveWhich of the following is not required for a UV-vis absorption spectrophotometer? Group of answer choices camera detector monochromator light sourceMy daughter have eye retina area is yellow. Please suggest biotechnological solution.Describe your methodology.
- When trying to focus an object on the microscope, you always begin with the ________________ objective lens and use __________________ adjustment knob to bring the object in focus. Once object is focused, high power objective lens can be used to magnify the object, and only use the ___________________ adjustment knob to focus at this point. a scanning; coarse; fine b low power; coarse; fine c low power; fine; coarse d scanning, fine; coarseIn light microscopy, when magnification is increased, the lens focuses closer to the sample. This makes the user more likely to ram the sample into the lens. Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm