You should always position the cells or tissues in the center of view before increasing magnification, because... You know that the image will move right as you move the slide to the left. You know that the image will move up as you move the slide down. You know that the center of the field of view will be unchanged. This is the best way to avoid breaking the slide with an objective

You should always position the cells or tissues in the center of view before increasing magnification, because... You know that the image will move right as you move the slide to the left. You know that the image will move up as you move the slide down. You know that the center of the field of view will be unchanged. This is the best way to avoid breaking the slide with an objective

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter15: Instrumentation In Medical Imaging

Section: Chapter Questions

Problem 9P

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You should always position the cells or tissues in the center of view before increasing magnification, because
Which of the following is true about the relationship between depth of field and magnification? There is no relationship. When magnification increases, depth of field decreases. When magnification increases, depth of field increases. Stereomicroscopes offer a lesser depth of field than compound microscopes. The depth of field of a microscope determines how much magnification it can achieve.
In light microscopy, when magnification is increased, the lens focuses closer to the sample. This makes the user more likely to ram the sample into the lens. Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?
What is the rule for determining total magnification? A. Ocular lens magnification + objective lens magnification = total magnification B. Ocular lens magnification - objective lens magnification = total magnification C. Ocular lens magnification x objective lens magnification = total magnification
Put the steps for using a microscope in order. While looking through the eyepiece, use the coarse adjustment knob to bring the object roughly into focus. Lower the stage as far away from the objectives as possible. Use the fine adjustment knob to bring the object into sharp focus. Turn on the light. Place the slide on the stage and center it. Turn the nosepiece to the lowest power objective.
You should always position the cells or tissues in the center of view before increasingmagnification, because.A.You know that the image will move right as you move the slide to the left.B. You know that the image will move up as you move the slide down.C.You know that the center of the field of view will be unchanged.D. This is the best way to avoid breaking the slide with an objective.
Why did we calculate, rather than measure, the diameter of the field of view on medium and high power? When using the compound microscope, you always begin viewing your specimen on __________ power, and use the __________ focus knob.
The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?
Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…
When trying to focus an object on the microscope, you always begin with the ________________ objective lens and use __________________ adjustment knob to bring the object in focus. Once object is focused, high power objective lens can be used to magnify the object, and only use the ___________________ adjustment knob to focus at this point. a scanning; coarse; fine b low power; coarse; fine c low power; fine; coarse d scanning, fine; coarse
If the ocular lens of a microscope has 10X magnifying power, and the 40X objective lens is being used for observation, what is the total magnification for the viewed image? 10x 43x 1000x 400x
How can you improve contrast when viewing a specimen?. What should you do if you have difficulty focusing in high power?. Why is important to use the fine adjustment nob only when focusing while using the high dry objective lens?