2. Mutations in bacterial promoters may increase or decrease the level of gene transcription. The consensus sequence for the -10 region of bacterial promoters is TATAAT. Which of the following variants would you expect to be the most deleterious to transcription; which might be the least deleterious? i. TATGTT ii. TATATT iii. TTTGTT iv. GGTGTT v. GATGAT

2. Mutations in bacterial promoters may increase or decrease the level of gene transcription. The consensus sequence for the -10 region of bacterial promoters is TATAAT. Which of the following variants would you expect to be the most deleterious to transcription; which might be the least deleterious? i. TATGTT ii. TATATT iii. TTTGTT iv. GGTGTT v. GATGAT

Biology: The Unity and Diversity of Life (MindTap Course List)

14th Edition

ISBN:9781305073951

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter10: Control Of Gene Expression

Section: Chapter Questions

Problem 5SQ

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2. What does an operon typically consist of? a. an operator, a promoter, and a cluster of genes b. a cluster of genes c. a promoter and a cluster of genes d. an operator and a cluster of genes e. a promoter and an operator
1. Why aren't ssb proteins necessary in transcription?
1. Which of the following conditions will result to deactivation of a gene? a. histone methylation b. phosphorylation of a transcription factor c. binding of hormones to its nuclear receptor d. deactivation of transcription factor inhibitory protein 2. Direct complementarity of the microRNA to the target mRNA will lead to a. mRNA degradation b. inhibition of transcription c. chromatin remodeling d. inhibition of translation
1. Transcription: a)State the role of RNA polymerase in gene transcription.b. Explain why the DNA is not used directly for protein translation (i.e., why is mRNA used instead?).c. Explain what occurs when a gene’s promoter region is open for RNA polymerase binding.d. Explain what occurs when a gene’s promoter regions is blocked from binding RNA polymerase.e. Explain how two cells, such as liver cells and skin cells, can become specialized in structure and function despite containing the same genome.
A binding .site for RNA polymerase is called a ___ . a. gene b. promoter c. codon d. protein
Three different bacteria species have the following consensus sequences upstream of a conserved gene. Species A Species B Species C -10 TAATAAT TTTAAT TATATT -35 TTGACA TTGGCC TTGAAA Table 15.2 Order the bacteria from most to least efficient initiation of gene transcription. A > B > C B > C > A C > B > A A > C > B
Binding of ______ to _______ in DNA can increase the rate of transcription of specific genes. a. activators; repressors b. activators; enhancers c. repressors; operators d. both a and b
A scientist compares the promoter regions of two genes. Gene A’s core promoter plus proximal promoter elements encompasses 70bp. Gene B’s core promoter plus proximal promoter elements encompasses 250bp. Which of the scientist’s hypotheses is most likely to be correct? More transcripts will be made from Gene B Transcription of Gene A involves fewer transcription factors Enhancers control Gene B’s transcription Transcription of Gene A is more controlled than transcription of Gene B.
Mechanisms that govern gene expression do not operate during ___ . a. transcription b. RNA processing c. translation d. knockouts
1. The following regulatory mechanisms occur before eukaryotic transcription EXCEPT a. the formation of the transcription factor complex. b. the phosphorylation of eukaryotic initiation factors. c. the regulation of nuclear localization of transcription factors. d. the alteration of the DNA-binding domain of the transcription factor.
1. In DNA amplification, using the Taq polymerase, what is the maximum number of amplification cycles? a. 30 b. 20 c. 60 d. 10 2. In the case of trp operon, for transcription to fully occur, which of the following conditions must be met? a. Environmental tryptophan level must be low. b. Environmental tryptophan must be absent. c. Environmental tryptophan level must be abundant. d. Both a and b are correct.
1. If a mutation occurs in a coding region a. it will cause a change or changes in the normal (wild type) amino acid sequence. b. the protein may not form correctly and as a result does not perform the intended function. c. the type of mutation can be a missense mutation if there is some or limited protein function. d. the type of mutation can be a nonsense mutation if there is no protein function. e. all of the above 2. If a mutation occurs in a non-coding region: a. it will cause no change in the normal (wild type) amino acid sequence. b. the protein is unaffected and still functions correctly. c. the type of mutation is called a silent mutation. d. the protein may not form correctly and as a result does not perform the intended function. e. all of the above