2. Phoebe was tasked to ensure that an enzyme responsible for keeping her species from extinction remains biologically active. To do so, she needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory: Buffer System Weak Acid Component K₂ Conjugate Base Salt PAH Benzoic acid, C6H5COOH 6.25 x 10-5 NaCH₂COO SAD Boric acid, H3BO3 5.81 x 107 NaH2BO3 DOH Hydrogen cyanide, HCN 6.20 X 10-10 NaCN A. In what buffer system (PAH, SAD, or DOH) will Phoebe store the enzyme? In your solution sheet, show calculations and briefly explain to support your answer. B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet C. Calculate the amount (in mL) of the 2.00 M stock solution (pH 10.00) of the same buffer system needed to prepare 250 mL of a 0.650 M buffer pH 10.00.
2. Phoebe was tasked to ensure that an enzyme responsible for keeping her species from extinction remains biologically active. To do so, she needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory: Buffer System Weak Acid Component K₂ Conjugate Base Salt PAH Benzoic acid, C6H5COOH 6.25 x 10-5 NaCH₂COO SAD Boric acid, H3BO3 5.81 x 107 NaH2BO3 DOH Hydrogen cyanide, HCN 6.20 X 10-10 NaCN A. In what buffer system (PAH, SAD, or DOH) will Phoebe store the enzyme? In your solution sheet, show calculations and briefly explain to support your answer. B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet C. Calculate the amount (in mL) of the 2.00 M stock solution (pH 10.00) of the same buffer system needed to prepare 250 mL of a 0.650 M buffer pH 10.00.
Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter15: Additional Aqueous Equilibria
Section: Chapter Questions
Problem 109QRT: The grid has six lettered boxes, each of which contains an item that may be used to answer the...
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