2. You are excited because you discovered a DNA sequence motif that appears to be in the promoters of very highly expressed genes. You suspect that it is the binding site for a transcription factor (TF). The problem is, that you can't identify the TF! The motif you found is: A-A-T-C-G-C/G-A/T-A/T-C/G-A/T where 'X' is any nucleotide and '/' denotes either of the two nucleotides. a) You give up trying to identify the TF and decide that you are going to engineer your own TF. How might you go about synthetically creating a novel TF that best targets this sequence? What considerations would you need to take into account here? b) How might you go about confirming that this sequence is indeed important for gene expression?

2. You are excited because you discovered a DNA sequence motif that appears to be in the promoters of very highly expressed genes. You suspect that it is the binding site for a transcription factor (TF). The problem is, that you can't identify the TF! The motif you found is: A-A-T-C-G-C/G-A/T-A/T-C/G-A/T where 'X' is any nucleotide and '/' denotes either of the two nucleotides. a) You give up trying to identify the TF and decide that you are going to engineer your own TF. How might you go about synthetically creating a novel TF that best targets this sequence? What considerations would you need to take into account here? b) How might you go about confirming that this sequence is indeed important for gene expression?

Name: 2. You are excited because you discovered a DNA sequence motif that a
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Description: 2. You are excited because you discovered a DNA sequence motif that appears to bein the promoters of very highly expressed genes. You suspect that it is the bindingsite for a transcription factor (TF). The problem is, that you can't identify the TF!

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter16: Regulation Of Gene Expression

Section: Chapter Questions

Problem 6TYK

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