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22-A. Consider a chromatography experiment in which two compo- nents with retention factors k = 4.00 and k2 = 5.00 are injected into a column with N = 1.00 × 10° theoretical plates. The retention time for the less-retained component is t,1 = 10.0 min. (a) Calculate fm and t,2. Find wy2 (width at half-height) and w (width at the base) for each peak.

22-A. Consider a chromatography experiment in which two compo- nents with retention factors k = 4.00 and k2 = 5.00 are injected into a column with N = 1.00 × 10° theoretical plates. The retention time for the less-retained component is t,1 = 10.0 min. (a) Calculate fm and t,2. Find wy2 (width at half-height) and w (width at the base) for each peak.

Biochemistry
Biochemistry
9th Edition
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Chapter2: Water: The Solvent For Biochemical Reactions
Section: Chapter Questions
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22-A. Consider a chromatography experiment in which two compo- nents with retention factors k, = 4.00 and k2 = 5.00 are injected into a column with N = 1.00 × 10° theoretical plates. The retention time for the less-retained component is t,1 = 10.0 min. (a) Calculate fm and 1,2. Find w12 (width at half-height) and w (width at the base) for each peak. (b) Using graph paper, sketch the chromatogram analogous to Figure 22-7, supposing that the two peaks have the same amplitude (height). Draw the half-widths accurately.
Transcribed Image Text:22-A. Consider a chromatography experiment in which two compo- nents with retention factors k, = 4.00 and k2 = 5.00 are injected into a column with N = 1.00 × 10° theoretical plates. The retention time for the less-retained component is t,1 = 10.0 min. (a) Calculate fm and 1,2. Find w12 (width at half-height) and w (width at the base) for each peak. (b) Using graph paper, sketch the chromatogram analogous to Figure 22-7, supposing that the two peaks have the same amplitude (height). Draw the half-widths accurately.
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