pH 12 10 10 12 Figure 1. Titration Curve of Lysine Table 2. pka Values and Identity of Lysine Experimental Theoretical % Error pKai pKa2 pKas pl
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Please answer the pKa Values and Identity of Lysine
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How was the theoretical value of the pKa determined? In that given graph.
by Bartleby Expert- After purifying the expressed protien His6-NEP of interest, the different fractions collected will be run on an SDS-Page gels. If our purification was successful a band of correct size should be present in: A) orginal lysate B) lysate flowthrough C) wash flowthrough D) Elute E) orginal lystae and elite fractions6. assay kits that determine the amount of glucose presentFour cosmid clones, which we will call cosmids A, B, C, and D, werehybridized to each other in pairwise combinations. The insert size ofeach cosmid was also analyzed. The following results were obtained:
- 1. How many TSS sites were identified using this technique? 2. Look at the labels next to each of the annotated TSSs. What are the labels for the TSS sites? 3. What is the coordinate for TSS_tra_16584216?8.Iron-uptake protein IupA is a human 302-aa, N-glycosylated, heterodimeric protein of considerable therapeutic interest. It contains 3 disulfide bridges that stabilise its structure. Explain what expression host you would use to produce the protein in recombinant form.complete steps of haworth projection of D-tagatose
- PLEASE MAKE THE DR BRUJIN GRAPH From these k-mers construct a de Bruijn graph and determine the sequence of the contig. AGCG ATCT ATGA ATGG ATTC CCCT CCTG CTCT CTGA CTGC CTTT GAAG GATT GCGT GCTC GTTC TATG TCAT TCTA TCTT TGAA TGAT TGGA TGTT TTCA TTCC TTTCFor each individual on gel, determine if the individual is homozygous for presence of the Alu insertion (++), heterozygous (+-), homozygous for the absence of the Alu insertion (- -), or if the results are inconclusive (I) (explain in detail what leads to your determination of each individual on the gel and why). Explain what may have caused any unexpected bands (neither 850 nor 550) lack of bands.1)De novo synthesis of nucleotides is an energy intensive process and therefore not always the primary source of nucleotides.Explain what is meant by this statement and how the process is regulated(specifically for urines)
- For each individual on each gel, determine if the individual is homozygous for presence of the Alu insertion(++), heterozygous (+-), homozygous for the absence of the Alu insertion (-), or if the results are inconclusive (I) (explain what makes you decide your determination, in detail). Explain what may have caused any unexpected bands (neither 850 nor 550) or lack of bands.Utilizing Bentham's "Felicific Calculus" step-by-step, give a justification in practicing proper use of facemasks outdoors as a health protocol during this time of COVID-19.A protein gives a single band on SDS gel electrophoresis, as shown inlanes 1 and 2 below. There is little, if any, effect from adding β-mercaptoethanol (BME) to the sample; if anything, the protein runs a little bitslower. When treated with the proteolytic enzyme thrombin and electrophoresis in the absence of BME, the protein migrates a bitmore rapidly (lane 3). But if BME is present, two much more rapidlymigrating bands are found (lane 4). Explain these results in terms of amodel for the protein.
