-24. The following data are for a liquid chromatographic column: Length of Packing Flow rate VM Vs A B C D A chromatogram of a mixture of species A. B, C, and D provided the following data: Nonretained Retention Time, min 3.1 5.4 24.7 cm 0.313 mL/min 1.37 mL 0.164 mL 13.3 14.1 21.6 Width of Peak Base (W), min 0.41 1.07 1.16 1.72 Calculate (a) the number of plates from each peak. (b) the mean and the standard deviation for N. (c) the plate height for the column. A B

-24. The following data are for a liquid chromatographic column: Length of Packing Flow rate VM Vs A B C D A chromatogram of a mixture of species A. B, C, and D provided the following data: Nonretained Retention Time, min 3.1 5.4 24.7 cm 0.313 mL/min 1.37 mL 0.164 mL 13.3 14.1 21.6 Width of Peak Base (W), min 0.41 1.07 1.16 1.72 Calculate (a) the number of plates from each peak. (b) the mean and the standard deviation for N. (c) the plate height for the column. A B

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.14QAP

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A new method is proposed to simultaneously detectand separatefour foodadditives by reversed-phase liquid chromatography. The data were obtained using theliquid chromatographic column with a flow rate of 0.5 mL per min and with the followingparameters: Pore size 100 A Particle Size 3.5 Internal diameter 4.6 mm Length 150 mm The food additives found in the sample resulted in the following data: ( see image for table) a.Calculate the number of plates from each peak. b.Calculate the plate height for the column. c.Retention factor for each peak.
How the 2D Thin layer Chromatography separation processes are worked? Please shortly wtite at your own words. Answer should be to the point (5-6 lines maximum).
which of the four binary mobile phase compositions (mixing ratio 1: 1) shown below will have the highest solvent strength ε ° on a normal phase hplc column? silica is used as a stationary phase. pentane + toluene methanol + acetone pentane + ethyl ethanoate propan-2-ol + ethyl ethanoate also need explaination why other options are incorrect
Gas chromatography was used to analyse for residues of toxic solvents, including benzene and 1,2-dichloroethane (DCE), in a pharmaceutical preparation. The following conditions were used: Column: 30 m long capillary with polysiloxane stationary phaseCarrier gas: He at 35 cm s–1 constant flowOven program: 40 °C at t = 0, increasing at 10 °C min–1 to 240 °C at t = 20 min. Detector: flame ionisation (FID) Benzene eluted with retention time = 11.5 min, peak width = 0.26 min 1,2-dichloroethane (DCE) eluted with retention time = 11.8 min, peak width = 0.28 min (i) What are the strengths and limitations of the flame ionisation detector in this application, compared with other possible methods of detection? (ii) What is the resolution, RS, for the separation of benzene and DCE on this column? (iii) Suggest two ways by which the resolution of benzene and DCE might be improved. Explain your choices.
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What is the role of stationary phase and mobile phase in Chromatography? Please shortly write at your own words. (5-6 lines maximum ).
Internal standard. A mixture containing 12.8 mM analyte (X) and 44.4 mM standard (S) gave chromatographic peak areas of 306 for X and 511 for S. A second solution containing an unknown quantity of X plus 55.5 mM S had peak areas of 251 for X and 563 for S. Find [X] in the second solution.
hree components in a mixture have varying distribution constants between the mobile phase and the stationary phass One of the components has a very low distribution constant, Would this component likely elute first or last and how would you design an experiment or modify the components of the separation to make it perform in the opposite manner?
A gas chromatogram of a mixture of toluene and ethyl (a) Measure w1/2 for each peak to the nearest 0.1 mm. When the thickness of the pen trace is significant relative to the length being measured, it is important to take the pen width into account. It is best to measure from the edge of one trace to the corresponding edge of the other trace, as shown at the bottom of the left column. (b) Find the number of theoretical plates and the plate height for each peak.
Procedure: analytically transfer between 0.4053g of iron(III) chloride hexahydrate, FeCl 3•6 H2O (M = 270.2957g•mol-1), and dissolve in 5.5 mL distilled, deionized water. Support the tube on a stir plate and over a 15-minute period add, using the 10mL burette, 5mL of a nominal 600mM solution of 2,4 pentandione in methanol under constant stirring. To the resulting solution, add via a 10mL burette 3mL of a nominal 450mM sodium acetate solution stirring constantly. At this point, a red crystalline solid should begin to precipitate. Transfer and support the reaction vessel in the hot water bath and once the reaction mixture has reached a stable temperature in the desired range, maintain the reaction mixture at this temperature, under constant stirring, for a 15-minute period Initial weight of iron(III) chloride hexahydrate: 0.4053g The final weight of the reaction tris(acetylacetonate)iron(III): 19.6 mg 3. What is the charge on the metal in the starting material for your synthesized…
The following data give the recovery of bromide from spiked samples of vegetable matter, measuredusing a gas–liquid chromatographic method. The same amount of bromide was added to each specimen(Roughan, J.A., Roughan, P.A. and Wilkins, J.P.G., 1983, Analyst, 108: 742).Tomato: 777 790 759 790 770 758 764 ug/gCucumber: 782 773 778 765 789 797 782 ug/g(a) Test whether the recoveries from the two vegetables have variances which differ significantly.(b) Test whether the mean recovery rates differ significantly.
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