3). You mutagenize bacteria, plate them to the permissive temperature, then replica plate to the restrictive temperature. After lots of replica plating and mapping, you find mutants in dnaB. a). What is the phenotype of these mutants at permissive and non-permissive temperatures? Focus on the macroscopic phenotype (what happens at the level of cells) and not the molecular phenotype (which step in replication is compromised). b). Can you make constitutive mutants (the mutation is always active) in dnaB? Why or why not?
3). You mutagenize bacteria, plate them to the permissive temperature, then replica plate to the restrictive temperature. After lots of replica plating and mapping, you find mutants in dnaB. a). What is the phenotype of these mutants at permissive and non-permissive temperatures? Focus on the macroscopic phenotype (what happens at the level of cells) and not the molecular phenotype (which step in replication is compromised). b). Can you make constitutive mutants (the mutation is always active) in dnaB? Why or why not?
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter19: Genomes And Proteomes
Section: Chapter Questions
Problem 1TYK
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