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- A Grignard reaction of phenylmagnesium bromide with 3-pentanone gives 3-phenylpentan-3-ol as the major product. The crude product contains the 3-phenylpentan-3-ol product, unreacted 3-pentanone, and biphenyl (a side product). A developing solvent is found that separates the mixture into three spots on a silica gel TLC plate. Considering the functional groups present, predict which compounds would have the smallest and largest Rf values. Briefly justify your answer.Write the reaction involved in Ferrox Test. a. What is the species responsible? b. Why is phenol negative in Ferrox Test? Based on the theoretical result, what is the order of reactivity of primary, secondary, and tertiary alcohols in the Lucas Test? a. Lucas Reagent contains ZnCl2 in HCl. What is the role of ZnCl2?What is the order of reactivity of SN1 and SN2 of n-butyl chloride, n-butyl bromide, sec-butyl chloride, tert-butyl chloride and crotyl chloride. Why? Sn2- with NaI/acetone and Sn1- with AgNO3/ethanol
- Q1: Give one lysis buffer that is commonly used for western blotting experiments and include its components Q3: To make sure that you used a similar amount of samples, what important step should be done before proceeding the electrophoresis stage? Q4. Why is it necessary to store the prepared lysates in a very low temperature?Why was it important to keep the dioxolane derivative (an acetal) cold and dry after it was formed?Solvent #1 is a 50:50 mixture of hexane and ethyl acetate and solvent #2 is 25% hexane. Which solvent mixture is more polar: the 50:50 mixture or the 25:75 mixture? Explain briefly What would happen to the Rf of the spots in solvent #2 compared to solvent #1? Why?
- How does washing with 10% sodium bicarbonate help to isolate ethyl cinnamate in each synthesis reaction? As part of your answer show the reaction of aqueous sodium bicarbonate with cinnamic acid and with cinnamoyl chloride (remembering that cinnamoyl chloride hydrolyzes to cinnamic acid in water).Describe how the product is purified. 4,4'-DIBROMOBIPHENYL [Biphenyl, 4,4'-dibromo-] Submitted by Robert E. Buckles and Norris G. Wheeler1. Checked by R. S. Schreiber, Wm. Bradley Reid, Jr., and Robert W. Jackson. 1. Procedure In a 15-cm. evaporating dish is placed 15.4 g. (0.10 mole) of finely powdered biphenyl (Note 1). The dish is set on a porcelain rack in a 30-cm. desiccator with a 10-cm. evaporating dish under the rack containing 39 g. (12 ml., 0.24 mole) of bromine. The desiccator is closed, but a very small opening is provided for the escape of hydrogen bromide (Note 2). The biphenyl is left in contact with the bromine vapor for 8 hours (or overnight). The orange solid is then removed from the desiccator and allowed to stand in the air under a hood for at least 4 hours (Note 3). At this point, the product weighs about 30 g. and has a melting point in the neighborhood of 152°. The crude 4,4'-dibromobiphenyl is dissolved in 75 ml. of benzene, filtered, and cooled to 15°. The…what should be the difference in the IR for the 2-naphthol and the product of the williamson ether synthesis? How could you use the IR to determine if the reaction was successful
- a. What is the purpose of adding Sodium carbonate to the black tea? You can explain its purpose as a paragraph or by showing the reaction of one phenolic compound reacting with it.b. Do you expect this phenolic compound to be soluble in water or DCM?2. In this experiment, you used ethyl acetate as the initial solvent to dissolve your mixture of benzoic acid, 4-bromoacetophenone and ethyl 4-aminobenzoate. i) Explain 3 reasons why ethyl acetate a good choice for this process? ii) Would you have obtained similar results with A) chloroform, B) ethanol, or C) water? Why or why not? Explain. Hint: Think about both solubility and miscibility of solvents when answering your question.In a hurry to complete the synthesis, Bill used 6M HCl, also on the reagent shelf, instead of the 6M H2SO4. As a result, describe what observation would he expect. Andrea used too much sulfuric acid. What observation would she expect? Explain. Explain why the alum crystals are washed free of impurities with the ethanol-ice-water mixture rather than with room-temperature deionized water.