5. You find a mutant with a 10-bp insertion between the (+1) and the upstream promotor sequences. This insertion is composed only of C and G nucleotides. 5a) Choose the best answer (1-4) below. This mutation will change 1. The sequence of the MRNA 2. The sequence of the protein 3. Both the sequences of the MRNA and the protein 4. Neither sequence will be changed 5b) Explain briefly your reasoning for your choice in (5a) above

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5. You find a mutant with a 10-bp insertion between the (+1) and the upstream promotor sequences. This insertion is composed only of C and G nucleotides.
5a) Choose the best answer (1-4) below. This mutation will change
1. The sequence of the mRNA
2. The sequence of the protein
3. Both the sequences of the mRNA and the protein
4. Neither sequence will be changed
5b) Explain briefly your reasoning for your choice in (5a) above
Transcribed Image Text:5. You find a mutant with a 10-bp insertion between the (+1) and the upstream promotor sequences. This insertion is composed only of C and G nucleotides. 5a) Choose the best answer (1-4) below. This mutation will change 1. The sequence of the mRNA 2. The sequence of the protein 3. Both the sequences of the mRNA and the protein 4. Neither sequence will be changed 5b) Explain briefly your reasoning for your choice in (5a) above
Below is a portion of a bacterial chromosome that contains a gene. The promoter region and the +1 base pair are indicated, as well
as the polarity of the two DNA strands. Analyse this DNA fragment and answer the questions 1-5 that follow.
+1
-10
-35
3' TTGCATCCGAAACGTACGATCGATESGCCGACTTATTACGATCGGACTACTGCGTCGTAGC5'
5' AACGTAGGCTTTGCATGCTAGCTAGCCGGCTGAATAATGCTAGCCTGATCACGCAGCATCG3'
...
...
Provide a brief statement in the space provided
1. What is the name if the TATTA sequence at -10 ?
2. What is the purpose of the -10 and -35 sites
3. What do you understand by the +1 site
4. Write the letters for the bases of the first 6 nucleotides of the mRNA molecule transcribed from this gene. Indicate the 5' and 3' end in your answer
Transcribed Image Text:Below is a portion of a bacterial chromosome that contains a gene. The promoter region and the +1 base pair are indicated, as well as the polarity of the two DNA strands. Analyse this DNA fragment and answer the questions 1-5 that follow. +1 -10 -35 3' TTGCATCCGAAACGTACGATCGATESGCCGACTTATTACGATCGGACTACTGCGTCGTAGC5' 5' AACGTAGGCTTTGCATGCTAGCTAGCCGGCTGAATAATGCTAGCCTGATCACGCAGCATCG3' ... ... Provide a brief statement in the space provided 1. What is the name if the TATTA sequence at -10 ? 2. What is the purpose of the -10 and -35 sites 3. What do you understand by the +1 site 4. Write the letters for the bases of the first 6 nucleotides of the mRNA molecule transcribed from this gene. Indicate the 5' and 3' end in your answer
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