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- 8. when a soil whose exchange sites were saturated with H+ is tested for pH using a litmus paper, what is the most probable result? A. Red litmus paper turns blue B. blue litmus paper manifests a false positive C. red litmus paper manifests a false positive D. Blue litmus paper turns redCalculate the amount of protein (in mg) in Sample 1 if the measurement at A280 = 0.197, taking into account the dilution factor 100 per and total volume of extract 140mlSpecific rotation of L-alanine [(+)alanine) in water (at 25 °C) is +2.8. Suppose you have 2.00 g of mixture of L and D-alanine [(-)alanine]. You dissolved it in 10 mL of water, and measured rotation in 10 cm cell. The observed rotation was +0.12. Calculate the ee% for the mixture. How many grams of D- and L-alanine do you have in the sample?
- Calculate the extinction coeficcient where the concentration is in mg/ml and the path length is in cm. The molecular weight of A is 290 g/mol. Re calculate the extinction coefficient with the concentration in nM. Note the unit of the newly calculated extinction coefficient will contain a mM-1 term. Given: Concentration: 5 microgram path length: 1cm absorbance: 0.0554 Show the order of elution from a cation-exchange resin using a buffer pH of 4.5: aspartic acid, valine, arginine, and alanine. Provide a brief explanation.When acids are added to a solution, the pH should ___________ . a. decrease b. increase c. stay the same d. cannot tell without testing
- 1- Using the pH 12 of the buffer and the pKa = 12.32. What is the molar ratio of A- to HA. 2- What is the molar amount of A- and HA needed to prepare a 0.2 M buffer? 3- How many grams of A- and HA are needed to prepare 40 mal of the buffer? The molar mass of HA is 142g/mol The molar mass for A- is 380.1g/molCalculate the amount of protein (in mg) in Sample 1 if the measurement at A280 = 0.636, taking into account the dilution factor as per question 6 (100ul) and the total volume of extract as per question 4 (140ml). Give your answer as a number with no decimal places.The column efficiency is constant for a column that uses a cons tant eluent composition. What does this say about the peak width of slowly eluting compounds relative to those of rapidly eluting compounds?
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