6. You weigh 5 milligrams of protein and dissolve it in 1.0 mL of water to make a stock solution with a concentration of 10 mg/mL. You plan to load 10ug of protein in each lane on your SDS PAGE. To do this, you would like to have 10µg in 5 ul so that you can load 5 µl into each well. (5 μl is easy to pipette and fits into a well.) 6a. Using your 5 mg/mL stock solution, you wish to make 100μL of the correct concentration of protein to load. How do you make this working solution? (Yes- you would want this to be in 1x sample buffer, but that makes this question more complicated. So for this question just make the protein in water.) Volume of 10mg/ml Final Volume 100μL of protein 6b. This is a 1: dilution.

6. You weigh 5 milligrams of protein and dissolve it in 1.0 mL of water to make a stock solution with a concentration of 10 mg/mL. You plan to load 10ug of protein in each lane on your SDS PAGE. To do this, you would like to have 10µg in 5 ul so that you can load 5 µl into each well. (5 μl is easy to pipette and fits into a well.) 6a. Using your 5 mg/mL stock solution, you wish to make 100μL of the correct concentration of protein to load. How do you make this working solution? (Yes- you would want this to be in 1x sample buffer, but that makes this question more complicated. So for this question just make the protein in water.) Volume of 10mg/ml Final Volume 100μL of protein 6b. This is a 1: dilution.

Anatomy & Physiology

1st Edition

ISBN:9781938168130

Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble

Publisher:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble

Chapter2: The Chemical Level Of Organization

Section: Chapter Questions

Problem 12RQ: A substance formed of crystals of equal numbers of cations and anions held together by ionic bonds...

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