1.What is the molar concentration of a 15% glycerol solution? (Glycerol has a molecular weight of 92.1.) 2.How do you prepare 750 mL of 0.5X gel running buffer from a 5X stock solution?
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1.What is the molar concentration of a 15% glycerol solution? (Glycerol has a
molecular weight of 92.1.)
2.How do you prepare 750 mL of 0.5X gel running buffer from a 5X stock
solution?
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- 1) If you have 2.5g of hydrocortisone POWDER, how many grams of a 0.05% ointment can be compounded? 2) How many mL of a 0.45% saline solution can be made with 6750 mg of sodium chloride? 3) Your pharmacy receives an order to compound 120 g of a 1.25% cream. How many mg of drug are required to compound this order? 4) Is it possible to make 1200 mL of a 30% dextrose solution with only 75 g of dextrose? 5) You are asked to compound a 15% zinc oxide cream. How many grams of this cream can you make using 30,000mg of zinc oxide? 6) Using 45g of acetic acid, how many mL of a 20% acetic acid solution can be made? 7) Calculate the percent strength of a solution when 8 g of drug is dissolved in 400 mL of solution. 8) Calculate the percent strength of a solution when 2.5 g of drug is dissolved in 750 mL of solution. 9) Calculate the percent strength of a solution when 5000 mg of drug is dissolved in 500 mL of solution.1. What is the concentration of the solution prepared by diluting 50 µl of ampicillin (1.0mg/ml) to a final volume of 1ml? 2. Determine the volumes of phenol, chloroform, and isoamyl alcohol required to prepare250 ml of a 25:24:1 (v/v) solution.1. How many mL of distilled water must be added to make the Benzalkonium chloride preparation isotonic? 2. How many mL of 0.9% NaCl must be added to make a 100mL solution?
- 1. How do you prepare 25 mL of a 0.4X solution of SDS from a 20X stock?2. How do you prepare 50 mL of a 0.25X solution of Trisethylenediaminetetraacetic acid (TE) buffer from a 100X stock?6. Your pharmacy has on hand Robitussin CF Syrup (guaifenesin 100 mg/1 tsp).You receive a prescription for Robitussin CF Syrup and the patient is to receive 250 mg of guaifenesin q4h. How many milliliters will the patient receive in 1 dose? If you dispense a 4oz bottle of syrup, how many doses will the patient receive?1) A solution is made by combining 128 mL of ethanol and 0.452 L of water. In this solution, what is the solvent? What is the solute?2) If a solution contains 128 mL of ethanol and water to make 0.452 L of solution, what is the (v/v)% concentration?3) How many grams of sodium carbonate are needed to make 350 mL of a 0.5% (w/v) sodium carbonate solution?4) What is the molarity of a magnesium chloride solution made by dissolving 8.7g of magnesium chloride in 2.45 L of water?5) An exercise in lab requires you to make 500 mL of a 2.3M beryllium sulfide solution. How many moles of beryllium sulfide will you need?6) How many grams of sodium chloride are needed to make 0.60L of a 2.3M sodium chloride solution?7) How hot must the air in a balloon be heated if initially it has a volume of .750 L at 20°C and the final volume be 1.00 L?8) Convert 455 mmHg to atm.9) A container of gas has an initial pressure of 0.8 atm. The pressure is increased to 1.1atm and the final volume of gas is 2.3 liters.…
- 2 if there is a stock solution of 2000 (ug/ml) , is it possible to use SERIAL DILUTION to generate the following 8 tubes with their concentrations? A 2000 (ug/ml) B 1500 (ug/ml) C 1000 (ug/ml) D 750 (ug/ml) E 500 (ug/ml) F 250 (ug/ml) G 125 (ug/ml) H 0 (ug/ml)9. Find the final concentration as a percent (w/v) of dextrose when 75 mL dextrose 50 % (w/v), 50 mL of sodium chloride 0.9 % (w/v), and 100 mL of dextrose 5 % (w/v) sodium chloride 0.45 % (w/v) solution are mixed together.3. How will you prepare 500 ml of 0.750 M citrate buffer with pH of 4.25 from solid citric acid (C6H8O7) and solid sodium citrate (Na3C6H5O7). The Ka of citric acid is 7.40 x 10-4 . Given: • (Na3C6H5O7) solid sodium citrate = conjugate base in molarity [? −] • (C6H8O7) solid citric acid = weak acid in molarity [??] • 7.40 × 10−4 ?? of citric acid = ??? (acid-dissociation constant) • 500 ml of 0.750 M citrate buffer
- pH 3.5 4.5 5.5 6.5 7.5 8.5 Absorbance 0.098 0.027 0.068 0.028 0.032 0.054 Concentration in diluted supernatant (mg/ml) 0.196 0.054 0.136 0.0056 0.0064 0.0108 Concentration in undiluted supernatant (mg/ml) 0.98 0.27 0.68 0.28 0.32 0.54 Formula for the amount (g) of soluble proteins in the soy flour extract: In 15 ml of soy flour extract (with 1/50 dilution), Soluble protein (g) = (C x V x F) / 1000 C = concentration (mg/ml); F = dilution factor; V = volume of solution (ml) Calculate the % solubility of protein in the soy flour (Assume the soy flour contains 35% protein (w/w))pH 3.5 4.5 5.5 6.5 7.5 8.5 Absorbance 0.098 0.027 0.068 0.028 0.032 0.054 Concentration in diluted supernatant (mg/ml) 0.196 0.054 0.136 0.0056 0.0064 0.0108 Concentration in undiluted supernatant (mg/ml) 0.98 0.27 0.68 0.28 0.32 0.54 Formula for the amount (g) of soluble proteins in the soy flour extract: In 15 ml of soy flour extract (with 1/50 dilution), Soluble protein (g) = (C x V x F) / 1000 C = concentration (mg/ml); F = dilution factor; V = volume of solution (ml) Calculate the amount of soluble protein (in grams) in the soy flour extract1. Why is oil liquid at room temperature, while butter is solid? 2. Which of the following lipid sample is soluble or miscible in water? Explain. a. Oil b. Butter 3. Which lipid sample mentioned in number 2 is soluble or miscible in 70% ethanol? Explain. Do you think the solubility of the lipid samples will change if 100% ethanol is used instead?