A 1 mL sample of glycogen was calculated to contain 35 µmol (micromole) glucose. To 1 mL of this sample was added 2 mL of 2 M HCL. It was then hydrolysed by boiling the solution for 15 minutes. After boiling the hydrolysate was cooled and made up with H2O to a final volume of exactly 10 mL. The glucose was measured in this solution and found to have a concentration of 570 µg/mL (microgram/milliliter). i) Calculate the mass (mg) of glucose in the 10 mL of hydrolysate. As the 1mL of glycogen sample was made up to a final volume of 10 mL, this mass of glucose was produced by the hydrolysis of the original 1 mL glycogen sample. ii) Calculate the amount (µmol) of glucose produced by the hydrolysis of the glycogen sample. iii) Calculate the purity of the glycogen used in the sample as % Purity = (moles of measured glucose/ moles of calculated glucose in glycogen) *100 iv) state your answer in a complete sentence.
A 1 mL sample of glycogen was calculated to contain 35 µmol (micromole) glucose. To 1 mL of this sample was added 2 mL of 2 M HCL. It was then hydrolysed by boiling the solution for 15 minutes. After boiling the hydrolysate was cooled and made up with H2O to a final volume of exactly 10 mL. The glucose was measured in this solution and found to have a concentration of 570 µg/mL (microgram/milliliter). i) Calculate the mass (mg) of glucose in the 10 mL of hydrolysate. As the 1mL of glycogen sample was made up to a final volume of 10 mL, this mass of glucose was produced by the hydrolysis of the original 1 mL glycogen sample. ii) Calculate the amount (µmol) of glucose produced by the hydrolysis of the glycogen sample. iii) Calculate the purity of the glycogen used in the sample as % Purity = (moles of measured glucose/ moles of calculated glucose in glycogen) *100 iv) state your answer in a complete sentence.
General Chemistry - Standalone book (MindTap Course List)
11th Edition
ISBN:9781305580343
Author:Steven D. Gammon, Ebbing, Darrell Ebbing, Steven D., Darrell; Gammon, Darrell Ebbing; Steven D. Gammon, Darrell D.; Gammon, Ebbing; Steven D. Gammon; Darrell
Publisher:Steven D. Gammon, Ebbing, Darrell Ebbing, Steven D., Darrell; Gammon, Darrell Ebbing; Steven D. Gammon, Darrell D.; Gammon, Ebbing; Steven D. Gammon; Darrell
Chapter24: Polymer Materials: Synthetic And Biological
Section: Chapter Questions
Problem 24.79QP
Related questions
Question
A 1 mL sample of glycogen was calculated to contain 35 µmol (micromole) glucose. To 1 mL of this sample was added 2 mL of 2 M HCL. It was then hydrolysed by boiling the solution for 15 minutes. After boiling the hydrolysate was cooled and made up with H2O to a final volume of exactly 10 mL. The glucose was measured in this solution and found to have a concentration of 570 µg/mL (microgram/milliliter).
- i) Calculate the mass (mg) of glucose in the 10 mL of hydrolysate. As the 1mL of glycogen sample was made up to a final volume of 10 mL, this mass of glucose was produced by the hydrolysis of the original 1 mL glycogen sample.
- ii) Calculate the amount (µmol) of glucose produced by the hydrolysis of the glycogen sample.
iii) Calculate the purity of the glycogen used in the sample as
% Purity = (moles of measured glucose/ moles of calculated glucose in glycogen) *100
- iv) state your answer in a complete sentence.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
Follow-up Questions
Read through expert solutions to related follow-up questions below.
Follow-up Question
For the percent purity section should the 35 be converted into moles of calculated glucose as it is currently in micromoles
Solution
by Bartleby ExpertKnowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.Recommended textbooks for you
General Chemistry - Standalone book (MindTap Cour…
Chemistry
ISBN:
9781305580343
Author:
Steven D. Gammon, Ebbing, Darrell Ebbing, Steven D., Darrell; Gammon, Darrell Ebbing; Steven D. Gammon, Darrell D.; Gammon, Ebbing; Steven D. Gammon; Darrell
Publisher:
Cengage Learning
Chemistry: The Molecular Science
Chemistry
ISBN:
9781285199047
Author:
John W. Moore, Conrad L. Stanitski
Publisher:
Cengage Learning
General Chemistry - Standalone book (MindTap Cour…
Chemistry
ISBN:
9781305580343
Author:
Steven D. Gammon, Ebbing, Darrell Ebbing, Steven D., Darrell; Gammon, Darrell Ebbing; Steven D. Gammon, Darrell D.; Gammon, Ebbing; Steven D. Gammon; Darrell
Publisher:
Cengage Learning
Chemistry: The Molecular Science
Chemistry
ISBN:
9781285199047
Author:
John W. Moore, Conrad L. Stanitski
Publisher:
Cengage Learning