A 12 kb linear DNA fragment is subject to single or double RE digest and agarose gel electrophoresis, to yield the gel profile shown below. The first lane contains the size marker (M). a) Explain how the name of the enzyme EcoRI is derived. b) How many sites are there for EcoRI and PvuII respectively on this DNA fragment? c) Use the sizes of the DNA bands on the gel to compile a restriction enzyme map of the DNA fragment. Indicate the positions of the restriction enzymes sites for EcoRI and PvuII on the map.

Essentials Health Info Management Principles/Practices
4th Edition
ISBN:9780357191651
Author:Bowie
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Chapter4: Introduction To The Patient Record
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A 12 kb linear DNA fragment is subject to single or double RE digest and agarose gel
electrophoresis, to yield the gel profile shown below. The first lane contains the size marker
(M).
a) Explain how the name of the enzyme EcoRI is derived.
b) How many sites are there for EcoRI and PvuII respectively on this DNA fragment?
c) Use the sizes of the DNA bands on the gel to compile a restriction enzyme map of the DNA
fragment. Indicate the positions of the restriction enzymes sites for EcoRI and PvuII on the
map.

M Undigested ECORI
control
PvuII
ECORI
+ PvuII
12000 bp
9000 bp
6000 bp
5000 bp
4000 bp
2000 bp
1000 bp
|
Transcribed Image Text:M Undigested ECORI control PvuII ECORI + PvuII 12000 bp 9000 bp 6000 bp 5000 bp 4000 bp 2000 bp 1000 bp |
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