Homework Help is Here – Start Your Trial Now!
Science
Biology
A) 43 14 When Friend erythroleukemic cells are incubated with sodium butyrate, they differentiate into nondividing, hemoglobin-synthesizing cells. Butyrate-induced differentiation is accompanied by accumulation of acety- lated forms of H3 and H4 histones, as can be seen most clearly for H4 in Fig- ure 4-16A. In principle, butyrate treatment could increase the activity of his- tone acetyl transferases (HATS) or decrease the activity of histone deacety- lase complexes (HDACs). To distinguish between these alternatives, HAT and HDAC activities were measured in the presence and absence of butyrate (Figure 4-16B and C). From these results decide how butyrate treatment causes accumulation of acetylated histones. butyrate 'H-acetylated histone @ (cpm, thousands) (B) N (C) -butyrate + butyrate AQ boiled 10 20 30 time (minutes) free 'H-acetate (cpm, thousands) S43210 boiled -butyrate +butyrate

A) 43 14 When Friend erythroleukemic cells are incubated with sodium butyrate, they differentiate into nondividing, hemoglobin-synthesizing cells. Butyrate-induced differentiation is accompanied by accumulation of acety- lated forms of H3 and H4 histones, as can be seen most clearly for H4 in Fig- ure 4-16A. In principle, butyrate treatment could increase the activity of his- tone acetyl transferases (HATS) or decrease the activity of histone deacety- lase complexes (HDACs). To distinguish between these alternatives, HAT and HDAC activities were measured in the presence and absence of butyrate (Figure 4-16B and C). From these results decide how butyrate treatment causes accumulation of acetylated histones. butyrate 'H-acetylated histone @ (cpm, thousands) (B) N (C) -butyrate + butyrate AQ boiled 10 20 30 time (minutes) free 'H-acetate (cpm, thousands) S43210 boiled -butyrate +butyrate

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Topic Video
Question
A) 43 14 When Friend erythroleukemic cells are incubated with sodium butyrate, they differentiate into nondividing, hemoglobin-synthesizing cells. Butyrate-induced differentiation is accompanied by accumulation of acety- lated forms of H3 and H4 histones, as can be seen most clearly for H4 in Fig- ure 4-16A. In principle, butyrate treatment could increase the activity of his- tone acetyl transferases (HATS) or decrease the activity of histone deacety- lase complexes (HDACs). To distinguish between these alternatives, HAT and HDAC activities were measured in the presence and absence of butyrate (Figure 4-16B and C). From these results decide how butyrate treatment causes accumulation of acetylated histones. butyrate (B) H-acetylated histone (cpm, thousands) N -butyrate + butyrate boiled 9 30 10 20 time (minutes) (C) C free 'H-acetate (cpm, thousands) S43210 boiled -butyrate +butyrate,
Transcribed Image Text:A) 43 14 When Friend erythroleukemic cells are incubated with sodium butyrate, they differentiate into nondividing, hemoglobin-synthesizing cells. Butyrate-induced differentiation is accompanied by accumulation of acety- lated forms of H3 and H4 histones, as can be seen most clearly for H4 in Fig- ure 4-16A. In principle, butyrate treatment could increase the activity of his- tone acetyl transferases (HATS) or decrease the activity of histone deacety- lase complexes (HDACs). To distinguish between these alternatives, HAT and HDAC activities were measured in the presence and absence of butyrate (Figure 4-16B and C). From these results decide how butyrate treatment causes accumulation of acetylated histones. butyrate (B) H-acetylated histone (cpm, thousands) N -butyrate + butyrate boiled 9 30 10 20 time (minutes) (C) C free 'H-acetate (cpm, thousands) S43210 boiled -butyrate +butyrate,
Expert Solution
steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Mitochondrial mutations
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS