Directions: Suspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. If desired the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri dishes.
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- What is the chemistry principle behind when (treatment: heavy cream) is subjected to room temperature? Why does beating time, stability (%drain) and volume foam (specific gravity) is important? see the data below Treatment (Heavy Cream) Beating Time (min) Stability (%drain) Volume of Foam (Specific Gravity) (Room Temperature) 20 No drain 0.94 Heavy Cream (chilled) 20 No drain 0.76 Heavy Cream with sugar and vanilla 20 No drain 0.88 Heavy Cream (over-whipped) 25 No drain 0.83Label the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the graph for the gel filtration chromatography using a column experiment.Which of the following can most likely increase the yield of recrystallized benzoic acid? CHOICES: A fluted filter paper was used during cold filtration. The receiving flask from hot filtration does not contain a small amount of solvent. Activated charcoal was not added to the solution. The solution was filtered immediately after hot filtration.
- 0.2 ml of culture . Please explain solution3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mLWhat is the chemistry principle behind when (treatment: heavy cream) is subjected to CHILLED TEMPERATURE? What is the effect in beating time, stability (%drain) and volume foam (specific gravity) is important? see the data below Treatment (Heavy Cream) Beating Time (min) Stability (%drain) Volume of Foam (Specific Gravity) (Room Temperature) 20 No drain 0.94 Heavy Cream (chilled) 20 No drain 0.76 Heavy Cream with sugar and vanilla 20 No drain 0.88 Heavy Cream (over-whipped) 25 No drain 0.83
- Explain this example of the following water quality serial dilution. Note the dilution factors and how you would calculate #’s from the incubated plates. ……A serial dilution yielded TNTC in the 1:1 plate and the 1:10 plate, 50 cfu’s in the 1:100 plate and 3 cfu’s in the 1:1000 plate . What is the concentration (CFU)/ml of the raw sample? Is water acceptable for potable or Recreational use? Explain. When do you use filtration rather than serial dilutionWhy are we using this materials and centrifuging at 15000 rpm? Lipid peroxidation experiment:Materials;• 10% TCA solution• 0.67% TBA solution• Tissue sample Procedures:The tissue sample is buffered at the ratio of 1 x 4 and crushed with the help of a blender. It is centrifuged at 15 000 rpm. Then, 2.5 mL of 10% TCA is added onto 0.5 mL of tissue sample and the tubes are mixed in vortex. It is kept in boiling water for 15 minutes and immediately cooled. After centrifugation at 5000 rpm for 15 minutes, 2 mL of each supernatant is transferred to another tube. 1 mL of 0.67% TBA is added on it and mixed in vortex. After the samples are kept in boiling water for 15 minutes and cooled immediately, their absorbance at 532 nm is recorded. The amount of MDA is calculated by utilizing the highest absorbance specific absorbance values (E = 1.56 x 105cm-1M-1 ) of the MDA-TBA complex formed at 532 nm.A solution is tested with three indicators and it has a pH of 9.8. Identify the colours of the following indicators: alizarin yellow R color:yellow,orange or red thymol blue: yellow,orange,blue or red bromothymol blue: yellow, green or blue
- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperPrepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:You carry out a dilution adding 20 mL of solution A and 80 mL of water. This dilution can NOT be expressed as: 1 in 4 dilution. 1:4 dilution. 5 fold dilution. having a dilution factor of 5. 1 in 5 dilution.