Directions: Suspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. If desired the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri dishes.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter1: The Clinical Laboratory
Section1.8: Laboratory Math And Reagent Preparation
Problem 15RQ
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Question: Determine the amount of dehydrated medium needed to prepare 15 butts, 20 slants, and 25 butt-slants. Include amount for 2 additional units of each as excess to compensate for compounding losses.

Directions: Suspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the
medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to
45-50°C. If desired the medium can be enriched with 5-10% blood or other biological fluids.
Mix well and pour into sterile Petri dishes.
Transcribed Image Text:Directions: Suspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. If desired the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri dishes.
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