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- When a mixture of various molecular sized dyes are run through a gel chromatography column, what is eluted in the Void Volume? A. Blue Dextran B. Flourescein C. Both D. NothingWith gels, we cannot directly identify the various bands without further analysis. However, we can make some assumptions off of their sizes. In order to determine their sizes, we need to add -?- to the gels. a ethidium bromide b loading dye c molecular markers d Coomassie blueA student performs a separation by TLC on a silica get plate that results in three spots. Ared spot is measured 23 mm away from the original sample spot, a blue spot is 42 mmaway, and a yellow spot is 49 mm away. The distance from the original spot to where thesolvent stopped is 55 mm.a) Calculate the Rf value for each component in the mixture. (Show your work and reportthe correct number of significant figures.) b) The silica gel plate is polar. Rank the three components in the mixture from lowest tohighest polarity. Explain your answer.
- #5.# a drug is order 100mg/m2 po BID. How many milliliters will a patient receive if her BSA is 1.80m2 and concentration of solutions is 100mg/5ml?Four known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?The elution of a protein with an isoelectric point of 7.5, is mostly likely to be affected by a change in pH from 7.4 to 6.9 in which type of protein separation technique? a.) size exclusion chromatography b.) SDS-acrylamide gel electrophoresis c.) affinity chromatography d.) ion exchange chromatography
- A. Among the elements from Group 1 to Group 3, select the 5 most medically-relevant elements. B. Rank them from 1 to 5, 1 being the most important and 5 being the least important. Explain. I will upvote, I promise pls plsIf a powder in a 2gm vial diluted with 6.8ml of diluent provides 8ml of solution and each ml contains 250mg of a drug, how many ml is needed to give 750mg.When bound to proteins, the Bradford reagent turns blue. You measured the absorbance of your samples at 595 nm, which corresponds to yellow light. Why did you measure the absorbance using yellow light instead of blue light?
- based on the table given, 1. what form of ILY is most present at pH4.0? a. ILY2- b. ILY1- c. ILY0 d. ILY1 e. ILY2+ 2. at pH9.0, at which electroed will ILY move? a. no movement b. anode c. cathode pls answer both. ill give thumbs up.What is the basis for the separation of proteins by the following techniques? (a) gel-filtration chromatography (b) affinity chromatography (c) ion-exchange chromatography (d) reverse phase HPLCDescribe step by step process of: a. Ion-exchange Chromatography b. Hydrophobic Interaction Chromatography c. Affinity Chromatography d. Reversed-Phase Chromatography e. Principle of Polyacrylamide Gel Electrophoresis f. Isoelectric Focusing g. Protein Sequencing