Salmonella enterica can grow on the lipid breakdown product ethanolamine as a sole carbon and nitrogen source. Ethanolamine is abundant in the mammalian gut, especially during inflammation. You hypothesize that S. enterica has genes encoding a pathway specifically required for growth on ethanolamine. Design a mutant hunt that would allow you to identify Salmonella genes necessary for growth on ethanolamine, and state: • the method of mutagenesis you will use (and why) • are you using a screen, a selection, and/or an enrichment to identify relevant mutants? • the independent and dependent variables • both positive and negative controls • potential outcomes of your experiment, and how you will interpret them

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.