A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution? b) How many cfu/mL were present in the original culture?
A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution? b) How many cfu/mL were present in the original culture?
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 2.4P
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A culture of Staphylococcus is diluted as follows:
(1) 20mL are added to 80mL of water.
(2) 10uL from (1) are then added to 9.99mL of water.
(3) A 10-2 dilution is made from tube # (2).
(4) 100uL from (3) are plated for a pour plate and incubated.
There were 34 colonies counted on one quarter of the plate following incubation.
a) What was the overall dilution?
b) How many cfu/mL were present in the original culture?
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