a) Eco01091 20/4 Plai 48 RatAPI 179 Autl 2617 Sapl 250 iel 183 Ehul, SspDI 235 Pdml 2234 Begl 2215 A2486 MCS Scal 2177 Tsol 2108 BsaXl 659 PUC18/19 2686 bp al 6a3 NmeAll 1822 A, Pscl s06 Gsul 1/84 Ctri0l 1A 66 Eco311 1/06 Eam105! 16M1 rep (PM81) Pap 1110 Vail 1217 Figure 1 (https://www.fishersel.com/shop/products/thermo-scientific-puc18-pue19-dna-50-g/fersdoost1) Figure I above is showing the plasmid map of PUCI8. Discuss how screening is carried out if this plasmid is used for cloning. Explain the THREE (3) important regions of this plasmid that enable it to work efficiently as b) a cloning vector? State Uhe differences between the plasmid in Figure 1 with PBR322 in terms of their sereening procedures. c) Discuss TWO (2) methods of transformation of competent bacterial cells with the plasmid in Figure 1. d)
a) Eco01091 20/4 Plai 48 RatAPI 179 Autl 2617 Sapl 250 iel 183 Ehul, SspDI 235 Pdml 2234 Begl 2215 A2486 MCS Scal 2177 Tsol 2108 BsaXl 659 PUC18/19 2686 bp al 6a3 NmeAll 1822 A, Pscl s06 Gsul 1/84 Ctri0l 1A 66 Eco311 1/06 Eam105! 16M1 rep (PM81) Pap 1110 Vail 1217 Figure 1 (https://www.fishersel.com/shop/products/thermo-scientific-puc18-pue19-dna-50-g/fersdoost1) Figure I above is showing the plasmid map of PUCI8. Discuss how screening is carried out if this plasmid is used for cloning. Explain the THREE (3) important regions of this plasmid that enable it to work efficiently as b) a cloning vector? State Uhe differences between the plasmid in Figure 1 with PBR322 in terms of their sereening procedures. c) Discuss TWO (2) methods of transformation of competent bacterial cells with the plasmid in Figure 1. d)
Chapter5: Unit, Percentage, Milliequivalent, Ratio, And Household Measures
Section: Chapter Questions
Problem 3.6P
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Question
c and d
Transcribed Image Text:a)
Eco01091 20/4 Plai 48
PaTAPI 179
Aatl 2617
el 183
Sepl 250
El:1, SspDl 235
Pdmi 2234
Begl 2215
2486
MCS
Scal 2177
Tsol 2108
BSAXI 659
PUC18/19
2686 bp
al 683
NmeAl 1822
Al, Pscl s06
Gsul 1/84.
Ctriol 1/9
rep tpMB1)
Cail 1217
Eco311 166.
Eam105! 16-1
Psp-i 1110
Figure 1
(https://www.fishersel.com/shop/products/thermo-scientific-puc18-pue19-dna-50-g/fersd0G51)
Figure I above is showing the plasmid map of PUCI8. Discuss how screening is carried out
if this plasmid is used for cloning.
Explain the THREE (3) important regions of this plasmid that enable it to work efficiently as
a cloning vector?
b)
State the differences between the plasmid in Figure 1 with PBR322 in terms of their
screening procedures.
c)
Discuss TWO (2) methods of transformation of competent bacterial cells with the plasmid in
Figure 1.
d)
bla (Ap)
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