a) In an experiment, you have changed a DNA sequence directly upstream from a start codon of an operon in E.coli. The reason is to investigate the function of this region of the DNA. The analysis revealed that after the changes, the same amount of mRNA is made from the operon, but very few proteins are being produced. What is the most likely DNA region that you have changed, and what is the function of this region? b) The lacZ gene, which encodes for the expression of ß-galactosidase during lactose metabolism, depends very much upon the presence or absence of lactose in the environment. In an experiment, you have introduced cellobiose, an analogue of lactose which also acts as an inhibitor of the enzyme, to study its effect on B-galactosidase activity. What do you foresee to observe, in relation to lactose metabolism?
a) In an experiment, you have changed a DNA sequence directly upstream from a start codon of an operon in E.coli. The reason is to investigate the function of this region of the DNA. The analysis revealed that after the changes, the same amount of mRNA is made from the operon, but very few proteins are being produced. What is the most likely DNA region that you have changed, and what is the function of this region? b) The lacZ gene, which encodes for the expression of ß-galactosidase during lactose metabolism, depends very much upon the presence or absence of lactose in the environment. In an experiment, you have introduced cellobiose, an analogue of lactose which also acts as an inhibitor of the enzyme, to study its effect on B-galactosidase activity. What do you foresee to observe, in relation to lactose metabolism?
Biology (MindTap Course List)
11th Edition
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Chapter14: Gene Regulation
Section: Chapter Questions
Problem 9TYU: A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous...
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