A mixture of three components is separated using paper chromatography, and each component is analyzed usingabsorption. With standards containing 100 ppm of each component run simultaneously, the following data wereobtained: cmpd A, 0.72 absorbance; B, 0.55; C, 0.80. For the unknown mixture, the data obtined when run under identicalconditions were as follows: cmpd A, .0.15 absorbance, B, 0.84; C, 0.75.a) Calculate the relative percentage composition of the unknown mixture.b) The distances traveled by the three components were 8.5, 6.3, and 5.5 cm, respectively.Sketch the appearance of the paper chromatogram. Label each spot.c) Calculate the Rf values if the solvent front is 18 cm.

A mixture of three components is separated using paper chromatography, and each component is analyzed using absorption. With standards containing 100 ppm of each component run simultaneously, the following data were obtained: cmpd A, 0.72 absorbance; B, 0.55; C, 0.80. For the unknown mixture, the data obtined when run under identic conditions were as follows: cmpd A, .0.15 absorbance, B, 0.84; C, 0.75. a) Calculate the relative percentage composition of the unknown mixture. b) The distances traveled by the three components were 8.5, 6.3, and 5.5 cm, respectively. Sketch the appearance of the paper chromatogram. Label each spot. e Colouloto the Df voluOc if the colvent front ic 1 O om

A mixture of three components is separated using paper chromatography, and each component is analyzed using absorption. With standards containing 100 ppm of each component run simultaneously, the following data were obtained: cmpd A, 0.72 absorbance; B, 0.55; C, 0.80. For the unknown mixture, the data obtined when run under identic conditions were as follows: cmpd A, .0.15 absorbance, B, 0.84; C, 0.75. a) Calculate the relative percentage composition of the unknown mixture. b) The distances traveled by the three components were 8.5, 6.3, and 5.5 cm, respectively. Sketch the appearance of the paper chromatogram. Label each spot. e Colouloto the Df voluOc if the colvent front ic 1 O om

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter15: Electrophoresis And Other Separation Methods

Section: Chapter Questions

Problem 5P

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A mixture of three components is separated using paper chromatography, and each component is analyzed using absorption. With standards containing 100 ppm of each component run simultaneously, the following data were obtained: cmpd A, 0.72 absorbance; B, 0.55; C, 0.80. For the unknown mixture, the data obtined when run under identical conditions were as follows: cmpd A, 0.15 absorbance, B, 0.84; C, 0.75. a) Calculate the relative percentage composition of the unknown mixture. b) The distances traveled by the three components were 8.5, 6.3, and 5.5 cm, respectively. Sketch the appearance of the paper chromatogram. Label each spot. c) Calculate the Rf values if the solvent front is 18 cm.
A new method is proposed to simultaneously detectand separatefour foodadditives by reversed-phase liquid chromatography. The data were obtained using theliquid chromatographic column with a flow rate of 0.5 mL per min and with the followingparameters: Pore size 100 A Particle Size 3.5 Internal diameter 4.6 mm Length 150 mm The food additives found in the sample resulted in the following data: ( see image for table) a.Calculate the number of plates from each peak. b.Calculate the plate height for the column. c.Retention factor for each peak.
Internal standard. A mixture containing 12.8 mM analyte (X) and 44.4 mM standard (S) gave chromatographic peak areas of 306 for X and 511 for S. A second solution containing an unknown quantity of X plus 55.5 mM S had peak areas of 251 for X and 563 for S. Find [X] in the second solution.
Internal standard (As , Cs ), analyte with known concentration (Ax , Cx ) In a chromatographic equipment, a solution containing 0.006727 X and 0.008331 M S give peak area of Ax= 2316 and As= 207. To analyze an unknown sample, 5.0mL of 0.008331M S was added to 5.0mL of X, and the mixture was diluted to 50.0mL. This mixture gave a chromatography spectrum with area Ax= 3206 and As= 200. Find Cx.