A phenylgalactoside-type compound, which yields galactose when enzymatically cleaved, was added as the sole carbon source to petri dishes containing E. coli culture medium. E. coli can utilize galactose for growth. Each of the following bacterial strains was plated on this medium and scored for growth after 1-2 days. Growth Genotype lacZ+ lacI+ lacz+ lacIs - lacz+ lacl- oc lacZ+ lacI+ + a) What do you conclude about this compound with respect to its ability to be cleaved by ß- galactosidase? Why? b) What do you conclude about this compound with respect to its ability to inactivate the Lac repressor? Why? +

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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A phenylgalactoside-type compound, which yields galactose when enzymatically cleaved,
was added as the sole carbon source to petri dishes containing E. coli culture medium. E.
coli can utilize galactose for growth. Each of the following bacterial strains was plated on
this medium and scored for growth after 1-2 days.
Growth
Genotype
lacz+ lacI+
lacz+ lacIs
lacz+ lacl-
+
oc lacZ+ lacI+
+
a) What do you conclude about this compound with respect to its ability to be cleaved by ß-
galactosidase? Why?
b) What do you conclude about this compound with respect to its ability to inactivate the
Lac repressor? Why?
T
Transcribed Image Text:A phenylgalactoside-type compound, which yields galactose when enzymatically cleaved, was added as the sole carbon source to petri dishes containing E. coli culture medium. E. coli can utilize galactose for growth. Each of the following bacterial strains was plated on this medium and scored for growth after 1-2 days. Growth Genotype lacz+ lacI+ lacz+ lacIs lacz+ lacl- + oc lacZ+ lacI+ + a) What do you conclude about this compound with respect to its ability to be cleaved by ß- galactosidase? Why? b) What do you conclude about this compound with respect to its ability to inactivate the Lac repressor? Why? T
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