You are tasked with cultivating the following microorganisms: A recombinant strain of E. coli A wild strain of Streptomyces clavuligerus • Suggest a storage method for each microorganism and justify your choice. You have at your disposal a refrigerator and an
You are tasked with cultivating the following microorganisms:
A recombinant strain of E. coli
A wild strain of Streptomyces clavuligerus
• Suggest a storage method for each microorganism and justify your choice. You have at your disposal a refrigerator and an ultrafreezer, in addition to materials and conditions for handling microorganisms.
• Propose a culture medium for each (choose a complex medium for one organism and a synthetic medium for the other)
As a complement of nutrients, they must be indicated on the following scale: +++ : above 5 g/L
++ : from 1 to 5 g/L
+ : below 1 g/L
• Describe the procedure for preparing and sterilizing the components of the culture media for a volume of 5.0 L
• Make a bench process selection scheme, up to the step of selecting a benchtop reactor 5 L.
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