A procedure called chromatin immunoprecipitation allows scientists to determine where a particular protein is located in the genome. You conduct this procedure for a human regulatory transcription factor and a histone acetyltransferase, and you find that the two proteins are present together at the promoters of many genes. Is the transcription factor more likely to be an activator or a repressor? Why?

A procedure called chromatin immunoprecipitation allows scientists to determine where a particular protein is located in the genome. You conduct this procedure for a human regulatory transcription factor and a histone acetyltransferase, and you find that the two proteins are present together at the promoters of many genes. Is the transcription factor more likely to be an activator or a repressor? Why?

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 8TYU: Through alternative splicing, eukaryotes (a) reinforce gene inactivation (b) prevent transcription...

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What role does an operator sequence serve in bacterial gene expression regulation? Describe one change in bacterial cells that can cause a repressor protein to go from inactive to active or from active to inactive.
Transcriptional regulation often involves a regulatory protein that binds to a segment of DNA and a small effector molecule that binds to the regulatory protein. Do each of the following terms apply to a regulatory protein, a segment of DNA, or a small effector molecule? A. Repressor B. Inducer C. Operator site D. Corepressor E. Activator F. Attenuator G. Inhibitor
A genon wide analyst of human genome indicated that approximately 1500 genes encoded transcription factors that are important for sequence specific gene expression. based on this information approximately what percentage of the protein coding genes in human belong to the transcription factors? A. 15% B. 5% C. 7.5% D.1.5%
Which of the following mutations could be appropriately describedas a position effect?A. A point mutation at the –10 position in the promoter regionprevents transcription. B. A translocation places the coding sequence for a muscle-specificgene next to an enhancer that is turned on in nerve cells.C. An inversion flips a gene from the long arm of chromosome 17(which is euchromatic) to the short arm (which isheterochromatic).
How do you think that transcription randomizes positions of nucleosomes and repression restores the ordering after transcription? How might you test to see if there was an exchange of histone subunits during transcription or if the nucleosome is truly transferred as a single unit? Would you expect the DNA band representing the distance from the restriction enzyme site to the hypersensitive site to be a single band or a smear? Defend your answer.
A gene, which we will call gene C, can be epigenetically modified in such a way that its expression in some cells is permanently silenced. Describe how you could conduct cell-fusion experiments to determine if a cis- or a trans-epigenetic mechanism is responsible for maintaining the silencing of gene C.
What are the functions of transcriptional activator proteins and repressor proteins? Explain how they work at the molecular level.
In 3-4 sentences eachExplain the difference between prokaryotic and eukaryotic transcription. Explain the difference between an activator and a repressor? Explain the difference between a promoter and an enhancer? Explain the difference between an intron and an exon? Explain what happens when eIF-2 is phosphorylated and when it is not phosphorylated?
How can methylation affect transcription? a. It may prevent the binding of regulatory transcription factors. b. It may enhance the binding of regulatory transcription factors. c. It may attract methyl-CpG-binding proteins, which inhibit transcription, to bind to a methylated sequence. d. All of the above are possible ways for methylation to affect transcription
How do I draw the sequence and explain the process for each step? Draw the sequence of molecular events that occurs to induce STAT transcription factor localization to the nucleus. To complete this, you will draw the first step that occurs, then draw a new figure with the second step that occurs, then draw a new figure with the third step that occurs, and so on until you have completed all of the steps. On the drawing, briefly label each molecular event (each drawing). For this brief label, explain what is happening during each step.
Refer to figure, which shows the distribution of histone H2A.Z on nucleosomes near a transcription start site. What experimental technique would have been used to generate the data for this figure? Briefly describe the operation of this technique.
Which of the following is true of CpG islands? a. They are methylated near promoters of actively transcribed genes. b. They are unmethylated near promoters of actively transcribed genes. c. Acetylation of CpG islands leads to repression of transcription. d. CpG islands code for RNA molecules that activate transcription.