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- What is biochemical oxygen demand (BOD) test? At what stage of Sewage treatment this test is performed? BOD level of three samples of water labelled as A, B and C are 30 mg/L, 10mg/L and 500 mg/L respectively. Which sample of water is most polluted?You have been working in the quality control lab at Koo for a month when you noticed a dramatic increase in the incidence of thermophilic incubator spoilage of canned sweet corn produced by the company. Describe the steps you would use to determine the cause of the increased spoilage. Your discussion must include the procedures you would use to determine the thermal death time and the D-value of the spoilage organism.A 10-L stirred tank is used to cultivate mouse myeloma cells. Dry air is sparged from the bottom at 1.0 L/min to supply oxygen to the system. The gas composition at the outler is 16% oxygen, 3.5% CO2, 6.5% water vapor, and balanced nitrogen. The ambient pressure and temperature are 1 atm and 37 °C, respectively. The dissolved oxygen in the reactor is controlled at 20% of saturation with ambient air. a. Assuming the reactor is at a steady-state, what is the volumetric oxygen transfer coefficient (kLa)? b. The specific OUR of myeloma cells is 5*10-11 mmol/cell*h. What is the cell concentration? c. What is the respiratory quotinent (RQ) (i.e., mole CO2 produced/mol O2 consumed)? Assume that the mole fraction of CO2 in the ambient air is negligible. The universal gas constant R = 0.082 L*atm/mol*K
- The authors of this paper also tested another minimal media (Media C) which is exactly the same as Media B except that the pH is 7.4 instead of 7.1. Which media components would be different in Media C? For each component that is different, state if will be larger or smaller than the amount in Media B.A given municipal wastewater can be treated with the following methods. N + AS, Activated sludge with pre-denitrificationAS + N Activated sludge with post-denitrificationor UCT (Biological Phosphorus Purification designed at the University of Cape Town) Which process needs the greatest addition of lime? (choose the right answer) AS + N N + AS UCT Everyone needs the same amount of additivesDetermine the fraction of Vmax that would be obtained when the substrate concentration, [S], is 0.5Km. Vmax fraction: .769
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