A. Describe the role of both the light AND the DCPIP in this experiment. B. Calculate the rate of photosynthesis( absorption units/min) for sample 1 and 6
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A. Describe the role of both the light AND the DCPIP in this experiment.
B. Calculate the rate of photosynthesis( absorption units/min) for sample 1 and 6
Light is one of the main raw materials in the process of photosynthesis. All photosynthetic organisms contain photosynthetic pigments that can absorb light such as chlorophyll "a" and it absorbs light in violet-blue and reddish orange-red wavelengths. The amount of light absorbed by the pigments determines the rate of photosynthesis plants.
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- . Define the following terms:a. light-dependent reactionsb. light-independent reactionsc. chlorosomed. P680e. P700To assess photosynthesis, we relied heavily on a spectrophotometer to measure the color intensity of DCPIP in our solution. We set the spectrophotometer at wavelengths in the visible spectrum. What is the approximate wavelength range (in nm) for the visible spectrum? For example, the ultraviolet spectrum wavelength range is approximately 10-400 nmIn a photosynthetic investigation, a student measured the following pH of the stroma and the inner thylakoid compartments of spinach leaf cells at the conclusion of the experiment. Source of Fluid pH (mean) Standard Error of Mean (SEM) Stroma 8.2 0.75 Inner compartment of thylakoid 5.6 0.35 Describe the stages of photosynthesis. Explain the need for a constant supply of inorganic carbon dioxide.
- If a leaf containing the sameconcentration of chlorophyll as in the solution wasexposed to the same ultraviolet light, no fluorescencewould be seen. Propose an explanation for thedifference in fluorescence emission between thesolution and the leaf.Suppose a researcher is carrying out studies in which she adds a nonphysiological electron donor to a suspension of chloroplasts. Illumination of the chloroplasts yields oxidation of the donor. How could she tell whether photosystem I, II, or both are involved?Which of the following proteins is NOT involved in cyclic electron flow during the light reaction? A. ferredoxin B. plastocyanin C. photosystem I D. photosystem II
- An experiment was done to determine how color of light and distance affects photosynthetic activity of the pond weed. Pond weed was placed in a test tube containing a solution of sodium hydrogen carbonate, and had its end cut; light was placed 50 cm away and four colors were tested (clear, red, blue, and green). After placing the light, the pondweed was given a certain amount of time to adjust before counting the bubbles, bubbles were counted for 1, 3, and 5 minutes. Only the bubbles that came out of the cut end was counted to represent the rate of photosynthesis. Below is the tabulated results. What are the possible factors or errors that could have affected clear/white light to have lower photosynthetic activity? In theory this should have the highest rate.The lab instructor will have a photosynthesis demonstration set up for your observation. What product are we observing?At your work station, you have a spectrophotometer set at 600 nm to measure DCIP absorbance and four (4) test tubes containing different solutions. One of the test tubes is labelled “0” to blank/zero the spectrophotometer. The other three (3) test tubes contain a reaction mixture from a Hill reaction experiment using isolated chloroplasts and DCIP as an electron acceptor. DCIP is present in the oxidized state and exhibits a blue color with an absorbance at 600 nm. Upon receiving the electrons, DCIP is reduced to form DCIP-H2 that is colorless. These three (3) tubes, labelled “A”, “B”, and “C” were exposed to different light conditions during the Hill reaction. The Light conditions were random, including No light, Red light, and Green light. Please note that chlorophyll preferentially absorbs the light with wavelength of 660 nm (red) when compared to that of 520 nm (green). Your mission is to determine which Tube came from which experimental light condition. Tube: Tube…
- Visit the companion website at www.oup.com/us/mckeeto read the Biochemistry in Perspective essay entitledPhotosynthetic Studies. Then describe the Emersonenhancement effect. How was it used to demonstrate the existence of two different photosystems?At the beginning of the light reaction, light excites electrons. Explain.In what part of chloroplasts do the coupled redox reactions occur? stroma membrane thylakoid membrane intermembrane space thylakoid lumen stroma