A. Describe the role of both the light AND the DCPIP in this experiment.  B. Calculate the rate of photosynthesis( absorption units/min) for sample 1 and 6

Principles Of Radiographic Imaging: An Art And A Science
6th Edition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Chapter21: Computed Radiography
Section: Chapter Questions
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A. Describe the role of both the light AND the DCPIP in this experiment. 

B. Calculate the rate of photosynthesis( absorption units/min) for sample 1 and 6

A buffered solution containing chloroplasts and DCPIP was divided equally among six identical samples. The
samples were placed at various distances from a lamp, and then all samples were exposed to white light from the
lamp for 60 minutes at room temperature. Sample 3 was wrapped in foil to prevent any light from reaching the
solution. At 20-minute intervals, the photosynthetic activity in each sample was determined by measuring the
relative absorbance of 605 nm light. See the results of the experiment below
Relative Absorbance of 605 nm Light (arbitrary units)
Sample
0 min
60 min
Distance
from lamp
(cm)
20 min
40 min
1
15
0.89
0.61
0.34
0.04
2
30
0.90
0.67
0.41
0.14
3*
30
0.88
0.87
0.86
0.87
4
45
0.86
0.69
0.47
0.26
5
60
0.92
0.75
0.59
0.41
6
75
0.88
0.79
0.71
0.58
*Wrapped in foil
LO
Transcribed Image Text:A buffered solution containing chloroplasts and DCPIP was divided equally among six identical samples. The samples were placed at various distances from a lamp, and then all samples were exposed to white light from the lamp for 60 minutes at room temperature. Sample 3 was wrapped in foil to prevent any light from reaching the solution. At 20-minute intervals, the photosynthetic activity in each sample was determined by measuring the relative absorbance of 605 nm light. See the results of the experiment below Relative Absorbance of 605 nm Light (arbitrary units) Sample 0 min 60 min Distance from lamp (cm) 20 min 40 min 1 15 0.89 0.61 0.34 0.04 2 30 0.90 0.67 0.41 0.14 3* 30 0.88 0.87 0.86 0.87 4 45 0.86 0.69 0.47 0.26 5 60 0.92 0.75 0.59 0.41 6 75 0.88 0.79 0.71 0.58 *Wrapped in foil LO
Expert Solution
Step 1

Light is one of the main raw materials in the process of photosynthesis. All photosynthetic organisms contain photosynthetic pigments that can absorb light such as chlorophyll "a" and it absorbs light in violet-blue and reddish orange-red wavelengths. The amount of light absorbed by the pigments determines the rate of photosynthesis plants. 

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