A. Peptic Digestion TUBE SIZE of egg white Results 1 (реpsin) Purple solution Minimal decreased in size 2 (НC) No change in size Deep Purple solution 3(реpsin, HC) Size decreased greatly Blue solution 4(pepsin, Na;CO3) No change in size Deep Purple solution 5 PΕPΤΟΕ Deep Purple solution

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Chapter14: Cpt Surgery Iv
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Please explain why is that  the results of each test tube on peptic digestion specifically in protein digestion

Procedure:
A. Peptic digestion
1. Prepare 4 test tubes as follows:
Tube 1 - 3 ml pepsin
Tube 2 - 3 ml of 0.4% HCI
Tube 3 - 3 ml pepsin + 1.5 ml of 0.4% HCl
Tube 4 - 3 ml pepsin + 1.5 ml of 1% Na2CO3
2. Add equal slices of hard-boiled egg white to each tube.
3. Cover and incubate at 40°C for 2 hours.
4. Add 4 drops of toluene and store till the next lab period.
5. Determine the extent of digestion by visually noting the size of the hard-boiled
egg white in each tube.
6. Withdraw 1 ml of the supernatant liquid from each tube and place in 4
test tubes labelled tube la. 2a, 3a, 4a.
7. Neutralize tubes 2a& 3a with solid sodium carbonate.
8. To each supernatant liquid, add 1 ml of 10% NaOH and 2 drops of 1% CuS04.
Compare the colors obtained in the 4 tubes.
separate
Transcribed Image Text:Procedure: A. Peptic digestion 1. Prepare 4 test tubes as follows: Tube 1 - 3 ml pepsin Tube 2 - 3 ml of 0.4% HCI Tube 3 - 3 ml pepsin + 1.5 ml of 0.4% HCl Tube 4 - 3 ml pepsin + 1.5 ml of 1% Na2CO3 2. Add equal slices of hard-boiled egg white to each tube. 3. Cover and incubate at 40°C for 2 hours. 4. Add 4 drops of toluene and store till the next lab period. 5. Determine the extent of digestion by visually noting the size of the hard-boiled egg white in each tube. 6. Withdraw 1 ml of the supernatant liquid from each tube and place in 4 test tubes labelled tube la. 2a, 3a, 4a. 7. Neutralize tubes 2a& 3a with solid sodium carbonate. 8. To each supernatant liquid, add 1 ml of 10% NaOH and 2 drops of 1% CuS04. Compare the colors obtained in the 4 tubes. separate
A. Peptic Digestion
TUBE
SIZE of egg white
Results
1 (реpsin)
Minimal decreased in
Purple solution
size
2 (НCI)
No change in size
Deep Purple solution
3(реpsin, HC)
Size decreased greatly
Blue solution
4(реpsin, NazCO;)
No change in size
Deep Purple solution
5 PΕPΤΟNE
Deep Purple solution
Transcribed Image Text:A. Peptic Digestion TUBE SIZE of egg white Results 1 (реpsin) Minimal decreased in Purple solution size 2 (НCI) No change in size Deep Purple solution 3(реpsin, HC) Size decreased greatly Blue solution 4(реpsin, NazCO;) No change in size Deep Purple solution 5 PΕPΤΟNE Deep Purple solution
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