A. Which operons would be functional under these conditions? lac and trp B. What repressors would be made? no repressors made C. Which repressor(s) would be made in the inactive form? trpR D. Which repressor(s) would be made in the active form? no repressor E. Which repressor(s) under these conditions can bind the operator sequence ? trpR & lacl F. Which repressor(s) under these conditions cannot bind the operator sequence? trpR & lacl G. What effect does lactose have on the bacterial cell's lac operon? it induces the operon H. What does the absence of glucose do to the bacterial cell? it inhibits the lac operon I. You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon? the trp operon is inhibited J. What kind of regulation does lactose provide to the repressor? allosteric

A. Which operons would be functional under these conditions? lac and trp B. What repressors would be made? no repressors made C. Which repressor(s) would be made in the inactive form? trpR D. Which repressor(s) would be made in the active form? no repressor E. Which repressor(s) under these conditions can bind the operator sequence ? trpR & lacl F. Which repressor(s) under these conditions cannot bind the operator sequence? trpR & lacl G. What effect does lactose have on the bacterial cell's lac operon? it induces the operon H. What does the absence of glucose do to the bacterial cell? it inhibits the lac operon I. You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon? the trp operon is inhibited J. What kind of regulation does lactose provide to the repressor? allosteric

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 9TYU: A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous...

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You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions using the dropdown menus provided in Canvas. Which operons would be functional under these conditions? What repressors would be made? (NOTE: name the repressors using their gene names) Which repressor(s) would be made in the inactive form? Which repressor(s) would be made in the active form? Which repressor(s) under these conditions can bind the operator sequence?
You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions using the dropdown menus provided in Canvas. Which operons would be functional under these conditions? What repressors would be made? (NOTE: name the repressors using their gene names) Which repressor(s) would be made in the inactive form? Which repressor(s) would be made in the active form? Which repressor(s) under these conditions can bind the operator sequence? Which repressor(s)under these conditions cannot bind the operator sequence? What effect does lactose have on the bacterial cell’s lac operon? What does the absence of glucose do to the bacterial cell? You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon? What kind of regulation does lactose provide to an operon’s…
The symbols a, b, and c in the table below represent theE. coli lac system genes for the repressor (I ), the operator(O) region, and the β-galactosidase (Z), although notnecessarily in that order. Furthermore, the order inwhich the symbols are written in the genotypes is notnecessarily the actual sequence in the lac operon.Activity (+) or inactivity (−) of Z geneGenotype Inducer absent Inducer presenta- b+ c+ + +a+ b+ c- + +a+ b- c- - a+ b- c+/a- b+ c- + +a+ b+ c+/a- b- c- - +a+ b+ c-/a- b- c+ - +a- b+ c+/a+ b- c- + +a. Which symbol (a, b, or c) represents each of the lacgenes I, O, and Z ?b. In the table, a superscript minus sign on a gene symbolmerely indicates a mutant, but some mutant behaviorsin this system are given special mutant designations.Using the conventional gene symbols for the lac operon,designate each genotype in the table.
You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions Which operons would be functional under these conditions? 2. What repressors would be made? (NOTE: name the repressors using their gene names) 3.Which repressor(s) would be made in the inactive form? 4.Which repressor(s) would be made in the active form? 5.Which repressor(s) under these conditions can bind the operator sequence? 6.Which repressor(s)under these conditions cannot bind the operator sequence?
What would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)
What would happen if the operator sequence of the trp operon contained a mutation that prevented the repressor protein from binding to the operator? (Explain what would happen in both the presence and absence of tryptophan)
If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
The figure at the beginning of this chapter showsthe binding of both a Lac repressor tetramer and aCRP-cAMP complex to the regulatory region of thelac operon.a. What is the key feature of a regulatory protein suchas the Lac repressor or CRP that allows it to regulate specifically the genes or operons it is supposedto control?b. On the figure, show the positions of the followingcomponents: (i) A Lac repressor monomer; (ii) a Lacrepressor dimer; (iii) all four DNA binding domains ofthe Lac repressor tetramer; (iv) a single helix-turnhelix motif; (v) the o1 part and either the o2 or o3 partsof the lac operator (assume the operon would be transcribed from right to left on the figure); (vi) themultimerization domains of the four Lac repressormonomers; (vii) an inducer-interacting domain; (viii)the CRP-cAMP complex; and (ix) a DNA loop.c. What is the physical basis for the formation of theDNA loop shown in the figure?d. On the figure, show the position of two axes ofsymmetry in the sequence…
You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation a.Which operons would be functional under these conditions? b.What repressors would be made? (NOTE: name the repressors using their gene names) c.Which repressor(s) would be made in the inactive form? d.Which repressor(s) would be made in the active form? e.Which repressor(s) under these conditions can bind the operator sequence? f.Which repressor(s)under these conditions cannot bind the operator sequence?
What are the effects of the following conditions on Lac operon of bacteria? Please do not forget to mention about the role of repressor, activator, RNA polymerase in each case. Glucose is absent and lactose is present Glucose is present and lactose is present Glucose is present and lactose is absent
You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions I really need a in depth epxplantion for the queshtions to make sure i understand the answers a.What effect does lactose have on the bacterial cell’s lac operon? b.What does the absence of glucose do to the bacterial cell? c.You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon? d.What kind of regulation does lactose provide to an operon’s repressor?
If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?