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DNA Extraction Using household materials
- Wash the fruit.
- Place the fruit (e.g. banana) in a zip lock plastic bag and crush it with your fist.
- Add the one half teaspoon of salt and one tablespoon of dishwashing liquid to the mashed
fruit mixture in the bag, zip it up and squeeze it in your hands for 1 minute.
- Place the strainer over the small glass.
- Pour the mixture into the strainer. Filter the mixture into the glass.
- Slowly pour ice cold rubbing alcohol into the glass, until it is about half full.
- Let it stand until the alcohol will form a separate layer on top of the fruit filtrate.
- Keep the glass still at eye level; do not shake it. Watch what happens. DNA will begin to
a.What is the importance of centrifugation in step 3?
b.
Step by step
Solved in 2 steps
- DNA Extraction Using household materials Wash the fruit. Place the fruit (e.g. banana) in a zip lock plastic bag and crush it with your fist. Add the one half teaspoon of salt and one tablespoon of dishwashing liquid to the mashed fruit mixture in the bag, zip it up and squeeze it in your hands for 1 minute. Place the strainer over the small glass. Pour the mixture into the strainer. Filter the mixture into the glass. Slowly pour ice cold rubbing alcohol into the glass, until it is about half full. Let it stand until the alcohol will form a separate layer on top of the fruit filtrate. Keep the glass still at eye level; do not shake it. Watch what happens. DNA will begin to appear where t 9. Carefully Scoop out the DNA with the straightened end of the paper clip. Touch the DNA. he alcohol and filtrate layer meet Briefly answer the following questions A. Describe two reasons for DNA isolation B. In step 2, the lysis solution you added to your Eppendorf tube and placed in the warm…DNA EXTRACTION Materials: knife 1 cup of fruit Table salt clean piece of cloth or strainer for filtering resealable bag dishwashing liquid 70% rubbing alcohol (chilled) shot glass (or any transparent and narrow container resembling a test tube) How to do the extraction: Before you begin, make sure you have chilled your alcohol in the freezer for at least 3 hours. Room temperature alcohol will not work nearly as well as cold alcohol. Place about 50 mL of alcohol in the freezer to chill it and take it out only when you’re going to need it. (The alcohol will not actually freeze.) 1. The first thing you will need is a sample. Since DNA is found in all living…Evaluate the gel provided below. Use the notes to help you read the gel: DNA is loaded in the wells (little pockets) at the top. The wells are the dark spaces under the numbers. DNA moves from the wells into the gel. The smaller the DNA the more quickly it moves. The samples on the ends are DNA ladders. We know the sizes of each of those bands and can use them to estimate the size of the DNA in the patient samples. 1 2 3 1: DNA ladder 2: Positive control 3: Patient 1 sample 4: Patient 2 sample 5: Patient 3 sample 6: Negative Control 7: DNA ladder 300 bp 100 bp 1. Why are positive and negative controls needed for PCR (or any test)? 2. Initially, test kits sent out by the CDC were flawed. The negative control sometimes showed up as positive. What misdiagnosis would potentially occur with this mistake? (Over or underdiagnosis?) 3. The CDC ultimately said to use the test anyway. Why might this be the case, given the current situation? 4. How would you evaluate the results above? Do any of…
- Gel Electrophoresis is used often in forensics. Look at the following gel to the left. From the evidence DNA, which individual matches the DNA evidence left at the crime scene?You are provided with coiled DNA and plasmid DNA that you subject to gel electrophoresis. Draw this gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. Exact fragment sizes are not important.What is the size of the DNA sample based on the gel profile below? Make sure to indicate the units. Sample bp 6000 5000 2000 1000 500 250 100 50 20 M 2.35u 4.26u 5.47u 6.67u 7.88u 9.47u 10.68u 12.27u 2.67u 10.26u Dye front: 16u
- Complete this Master Mix table for 8 DNA samples, a positive control, negative control, and an extra reaction for pipetting error. Show your work. Master Mix Conc. Of Total μL µL/Rxn (total per tube) Final Number of Stock Conc. Reactions needed for solution master mix PCR buffer 50X 1X Water DNTP mix 100 mM 200 µM MgCl2 Forward 24 mM 2.5 mM 2 μΜ 0.1 μΜ primer Reverse 2 μΜ 0.1 μΜ primer Taq polymerase DNA 5 U/uL 0.03 U/ µL 1 μL 60 μL Total volume of the entire reaction (µL)DNA Cheek Cells Lab Identify the component of the solution which is the extracted DNADrag and drop the appropriate text in the gaps below. Firstly, the plasmid DNA in the E. coli is propagated through Then the bacterial cells are pelleted by centrifugation at 10,000 RPM. The media supernatant is removed and the bacterial cells are The bacterial cells are then lysed via |. Contaminating macromolecules such as protein and chromosomal DNA is removed by Overnight incubation of the bacterial culture washed in cell resuspension buffer addition of lysis buffer addition of neutralisation buffer washing of the spin column separation via agarose gel electrophoresis
- DNA Extraction Using household materials Wash the fruit. Place the fruit (e.g. banana) in a zip lock plastic bag and crush it with your fist. Add the one half teaspoon of salt and one tablespoon of dishwashing liquid to the mashed fruit mixture in the bag, zip it up and squeeze it in your hands for 1 minute. Place the strainer over the small glass. Pour the mixture into the strainer. Filter the mixture into the glass. Slowly pour ice cold rubbing alcohol into the glass, until it is about half full. Let it stand until the alcohol will form a separate layer on top of the fruit filtrate. Keep the glass still at eye level; do not shake it. Watch what happens. DNA will begin to appear where t 9. Carefully Scoop out the DNA with the straightened end of the paper clip. Touch the DNA. he alcohol and filtrate layer meet A. In step 4, isopropyl alcohol is used to precipitate out the DNA because it is not soluble. What does this mean? B. What role did the alcohol, detergent and salt play?…Copy and paste the link below and watch the video on Youtube and Answer the Questionshttps://www.youtube.com/watch?v=g-dNJdOvBM4 Polymerase Chain Reaction Questions: 1. What are the materials used for the polymerase chain reaction? 2. Draw a schematic diagram of the procedure in PCR. 3. Why is it important to design the primers at the start of the laboratory procedure? 4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer? 5. What is the use for magnesium chloride? 6. How much template DNA is added? What is the concentration of the primers? 7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature? 8. In this particular PCR experiment, how many cycles was used? 9. Can this PCR be used on its own to find out if a person has Covid or not on its own? Why or why not?Why PCR needs the following: DNA template Primers DNA polymerase enzymes dNTPs Buffer Mg2+