Absorbance 2.0 1.8- 16 1.4 1.2 1.0 ast 0.2 Aap The Ser Glu Cly Val Me Tye Waln Ha LA 7 8 9 10 11 12 13 14 15 16 Effunt, mL (Flow rate-12mL/hr) (a1) Why Asp preceeds Ser/Thr? (a2) Why Ser/Thr preceed Val/Met/Leu/llu (isoleucine)? (a3) Why Val/Met/Leu/llu (isoleucine) preceed Tyr/Phe? -CH₂-CH-CH₂-CH- $0₂ SO₂ Chemical structure of resin polymer

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter13: Dimensional Analysis/units Conversion
Section: Chapter Questions
Problem 38SST
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In ion-exchange chromatography, amino acids are separated on the basis of electro-
static interactions and hydrophobic interactions with the resin. The chemical structure of the resin pol-
ymer with a negatively charged sulfonic acid group is shown to the right. An elution profile for the amino
acids using this resin is given below. Based on the elution profile above, explain the following:

Absorbance
2.0
1.8-
1.6
1.0
0.3
0.5
0.0
0.2
Ap
The
Ser
Glu
Gly
|Val
Mes
Les
Tye
املات
Ha
LA
NH₂
7 8 9 10 11 12 13 14 15 16
Effunt, mL (Flow rate-12mL/hr)
(a1) Why Asp preceeds Ser/Thr?
(a2) Why Ser/Thr preceed Val/Met/Leu/llu (isoleucine)?
(a3) Why Val/Met/Leu/llu (isoleucine) preceed Tyr/Phe?
-CH₂-CH-CH₂-CH-
SO₂
Chemical structure of
resin polymer
SO
Transcribed Image Text:Absorbance 2.0 1.8- 1.6 1.0 0.3 0.5 0.0 0.2 Ap The Ser Glu Gly |Val Mes Les Tye املات Ha LA NH₂ 7 8 9 10 11 12 13 14 15 16 Effunt, mL (Flow rate-12mL/hr) (a1) Why Asp preceeds Ser/Thr? (a2) Why Ser/Thr preceed Val/Met/Leu/llu (isoleucine)? (a3) Why Val/Met/Leu/llu (isoleucine) preceed Tyr/Phe? -CH₂-CH-CH₂-CH- SO₂ Chemical structure of resin polymer SO
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