ACH ACH + ACH + ACH + 1 x 10-M 3 x 10-M Acetylcholine Alone Concentration [Molar] Homatropine Homatropine Homatropine 3 x 10-⁹M 3 x 109 3 +/- 1 6 x 10-9 8 +/- 3 No response 3 x 10-8 28 +/- 6 10 +/- 4 No response 6 x 10-8 38 +/-8 21 +/-9 5+/-2 No response 3 x 10-7 55 +/- 5 43 +/- 7 27 +/-9 10 +/- 4 6 x 107 56 +/- 5 52 +/-3 38 +/- 7 20 +/- 7 3 x 10-6 55 +/- 4 53 +/- 6 43 +/- 6 6 x 106 56 +/- 2 54 +/- 5
ACH ACH + ACH + ACH + 1 x 10-M 3 x 10-M Acetylcholine Alone Concentration [Molar] Homatropine Homatropine Homatropine 3 x 10-⁹M 3 x 109 3 +/- 1 6 x 10-9 8 +/- 3 No response 3 x 10-8 28 +/- 6 10 +/- 4 No response 6 x 10-8 38 +/-8 21 +/-9 5+/-2 No response 3 x 10-7 55 +/- 5 43 +/- 7 27 +/-9 10 +/- 4 6 x 107 56 +/- 5 52 +/-3 38 +/- 7 20 +/- 7 3 x 10-6 55 +/- 4 53 +/- 6 43 +/- 6 6 x 106 56 +/- 2 54 +/- 5
Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter31: Immunochemistry
Section: Chapter Questions
Problem 31.70P
Related questions
Question
Below are responses generated using the isolated guinea-pig ileum preparation to determine a pA2 value for the cholinergic antagonist homatropine.
Responses are expressed in millimetres +/- SDM (n = 4).
Plot two data graph on a sheet or excell and then determine by Schild analysis the pA2 value and Schild slope.
show calculations
What conclusions can you draw; does the experiment/data fulfil the
criteria for a valid Schild analysis?
![ACH
ACH +
ACH +
ACH +
1 x 10-M
3 x 10-M
Acetylcholine Alone
Concentration
[Molar]
Homatropine Homatropine Homatropine
3 x 10-⁹M
3 x 109
3 +/- 1
6 x 10-9
8 +/- 3
No response
3 x 10-8
28 +/- 6
10 +/- 4
No response
6 x 10-8
38 +/-8
21 +/-9
5+/-2
No response
3 x 10-7
55 +/- 5
43 +/- 7
27 +/-9
10 +/- 4
6 x 107
56 +/- 5
52 +/-3
38 +/- 7
20 +/- 7
3 x 10-6
55 +/- 4
53 +/- 6
43 +/- 6
6 x 106
56 +/- 2
54 +/- 5](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc504be15-4a2e-43e2-bac4-44543a772361%2F5551132a-67f0-499d-bc2d-5304eb0680d3%2Fd43z5sq_processed.png&w=3840&q=75)
Transcribed Image Text:ACH
ACH +
ACH +
ACH +
1 x 10-M
3 x 10-M
Acetylcholine Alone
Concentration
[Molar]
Homatropine Homatropine Homatropine
3 x 10-⁹M
3 x 109
3 +/- 1
6 x 10-9
8 +/- 3
No response
3 x 10-8
28 +/- 6
10 +/- 4
No response
6 x 10-8
38 +/-8
21 +/-9
5+/-2
No response
3 x 10-7
55 +/- 5
43 +/- 7
27 +/-9
10 +/- 4
6 x 107
56 +/- 5
52 +/-3
38 +/- 7
20 +/- 7
3 x 10-6
55 +/- 4
53 +/- 6
43 +/- 6
6 x 106
56 +/- 2
54 +/- 5
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