After completing affinity chromatography run, need to do an 9:1 dilution with PBS, 0.001% W/V poloxamer 188, pH 7.4 with the elution pool. If the eluate is 3.5column volume (CV) how much PBS do I need to make with 1.5% extra ?
After completing affinity chromatography run, need to do an 9:1 dilution with PBS, 0.001% W/V poloxamer 188, pH 7.4 with the elution pool. If the eluate is 3.5column volume (CV) how much PBS do I need to make with 1.5% extra ?
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter26: An Introduction To Chromatographic Separations
Section: Chapter Questions
Problem 26.13QAP
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Please correct answer and don't use hend raiting
![After completing affinity chromatography run,
need to do an 9:1 dilution with PBS, 0.001% W/V
poloxamer 188, pH 7.4 with the elution pool. If
the eluate is 3.5column volume (CV) how much
PBS do I need to make with 1.5% extra ?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fee01d345-39f7-446a-835e-814ed992a1f7%2F4211504e-9d6b-4bec-9869-1eca8f5e22c5%2Fgqvc8xb_processed.jpeg&w=3840&q=75)
Transcribed Image Text:After completing affinity chromatography run,
need to do an 9:1 dilution with PBS, 0.001% W/V
poloxamer 188, pH 7.4 with the elution pool. If
the eluate is 3.5column volume (CV) how much
PBS do I need to make with 1.5% extra ?
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