After investigation of a specimen under light microscope and electron microscope, numerous amounts of spherical shaped particles were seen in the electron microscope while this type of structure was not visible under the light microscope. Further characterization revealed that these particles lack cellular organelles (like the nucleus, mitochondria…). Considering the above circumstance, answer the following questions. (A) What do you think would be the size of the specimen? (B) What do you suspect these particles are most likely to be? (C) Would you define these particles as living? Why/why not-justify your answer briefly
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
After investigation of a specimen under light microscope and electron microscope, numerous amounts of spherical shaped particles were seen in the electron microscope while this type of structure was not visible under the light microscope. Further characterization revealed that these particles lack cellular organelles (like the nucleus, mitochondria…). Considering the above circumstance, answer the following questions.
(A) What do you think would be the size of the specimen?
(B) What do you suspect these particles are most likely to be?
(C) Would you define these particles as living? Why/why not-justify your answer briefly
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