After irradiating wild-type cells of Neurospora (a haploid fungus), a geneticist finds two leucine-requiringauxotrophic mutants. He combines the two mutants ina heterokaryon and discovers that the heterokaryon isprototrophic.a. Were the mutations in the two auxotrophs in the samegene in the pathway for synthesizing leucine or in twodifferent genes in that pathway? Explain.b. Write the genotype of the two strains according toyour model.c. What progeny and in what proportions would youpredict from crossing the two auxotrophic mutants?(Assume independent assortment.)

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter15: From Dna To Protein
Section15.1: The Connection Between Dna, Rna, And Protein
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After irradiating wild-type cells of Neurospora (a haploid fungus), a geneticist finds two leucine-requiring
auxotrophic mutants. He combines the two mutants in
a heterokaryon and discovers that the heterokaryon is
prototrophic.
a. Were the mutations in the two auxotrophs in the same
gene in the pathway for synthesizing leucine or in two
different genes in that pathway? Explain.
b. Write the genotype of the two strains according to
your model.
c. What progeny and in what proportions would you
predict from crossing the two auxotrophic mutants?
(Assume independent assortment.)

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