You have constructed a recombinant vector harbouring pyruvate kinase. Subsequently, you wish to introduce your recombinant vector into Arabidopsis plants, since the enzyme is essential for the production of oil in developing seeds of Arabidopsis thaliana and
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
You have constructed a recombinant vector harbouring pyruvate kinase. Subsequently, you
wish to introduce your recombinant vector into Arabidopsis plants, since the enzyme is
essential for the production of oil in developing seeds of Arabidopsis thaliana and oil crops.
Explain how could you produce a transgenic Arabidopsis plant harbouring your synthetic
gene by Agrobaterium tumefaciens-mediated transformation?
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