Please answer all 3 MCQ with proper explanation 42. Which of the following is a required step in PCRall that apply)a. an annealing step at 50 degrees so primers can bindb. an extension step where the polymerase copies thestrand (around 72 degrees)c. a denaturation step at approximately 98 degrees toseparate the 2 strandsd. a ligation step that ligates the primer to the growing strand.e. 20-30 cycles of denaturation, annealing, ligation andextension.43. When two carbohydrates are epimers:a. one is a pyranose, the other a furanoseb. one is an aldose, the other a ketosec. they differ in length by one carbond. they rotate plane-polarized light in the same directione. they differ only in the configuration around a chiralcarbon atom44. When the linear form of glucose cyclizes, the product isa(n)a. anhydrideb. glycosidec. acetald. Hemiacetale. disaccharide

PCR is a process for synthesising artificial genes using dNTPs, primers, Taq DNA polymerase, and…

all that apply) a. an annealing step at 50 degrees so primers can bind b. an extension step where the polymerase copies the strand (around 72 degrees) c. a denaturation step at approximately 98 degrees to arote the 2 strands

all that apply) a. an annealing step at 50 degrees so primers can bind b. an extension step where the polymerase copies the strand (around 72 degrees) c. a denaturation step at approximately 98 degrees to arote the 2 strands

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1TYK

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Abiogenesis

Question

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Please answer all 3 MCQ with proper explanation

42. Which of the following is a required step in PCR
all that apply)
a. an annealing step at 50 degrees so primers can bind
b. an extension step where the polymerase copies the
strand (around 72 degrees)
c. a denaturation step at approximately 98 degrees to
separate the 2 strands
d. a ligation step that ligates the primer to the growing

strand.
e. 20-30 cycles of denaturation, annealing, ligation and
extension.
43. When two carbohydrates are epimers:
a. one is a pyranose, the other a furanose
b. one is an aldose, the other a ketose
c. they differ in length by one carbon
d. they rotate plane-polarized light in the same direction
e. they differ only in the configuration around a chiral
carbon atom
44. When the linear form of glucose cyclizes, the product is
a(n)
a. anhydride
b. glycoside
c. acetal
d. Hemiacetal
e. disaccharide

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