Alyssa is trying to prepare 50 mL of MBP Purification Buffer which should have final concentration of 20 mM Tris (pH 7.4), 200 mM NaCl, and 1 mM EDTA. What volumes of each of the following components would need to be mixed together to prepare the 50 mL solution? a. 0.1 M Tris (pH 7.4 b. 4 M NaCl c. 0.5 M EDTA d. DI water

Alyssa is trying to prepare 50 mL of MBP Purification Buffer which should have final concentration of 20 mM Tris (pH 7.4), 200 mM NaCl, and 1 mM EDTA. What volumes of each of the following components would need to be mixed together to prepare the 50 mL solution? a. 0.1 M Tris (pH 7.4 b. 4 M NaCl c. 0.5 M EDTA d. DI water

Chemistry & Chemical Reactivity

10th Edition

ISBN:9781337399074

Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Chapter4: Stoichiometry: Quantitative Information About Chemical Reactions

Section4.9: Spectrophotometry

Problem 3.1ACP

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