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Which of the following reagents is NOT correct reagents for the Sanger method of DNA sequencing?
All four dNTPs
a small amount of specific ddNTP
DNA Polymerase
DNA template to be sequenced
Pair of primers
Step by step
Solved in 2 steps with 2 images
- Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures? a. Chain termination occurs during synthesis of a new DNA strand. b. Many steps can be automated. c. No DNA is synthesized in the procedure. d. Fluorescent molecules can be used to detect the DNA.Which of the following reagents is NOT required for a PCR? a. DNA template b. DNA Polymerase c. All four dNTPs d. small amount of ddNTPs e. Pair of primersThe following question is related to Restriction Enzymes and RFLP. Using EcoRl, how many cuts were performed on DNA sequence A? A.) 2 B.) 3 C.) 4
- For DNA amplification using PCR to occur, which of the following are needed? A. DNA primers B. thermo stable DNA polymerase C. Helicase D. Choices A and BDuring a PCR reaction, all the following events occur except: A. Hybridization B. Binding of a 3’OH group in the active site of DNA polymerase C. Addition of nucleotide monomers on the 3’ end of the template DNA D. DNA denaturation E. Semi conservative replicationWhich of the following statements is true of a Sanger sequencing reaction but not colony PCR? Select all that apply. Must contain only a single primer ddNTPs are added Uses a heat stable DNA polymerase Requires a pair of primers that bind to opposite strands Requires temperature cycling
- When we discuss PCR and other similar techniques, the term Tm is often used. This refers to a.The temperature where the DNA molecules denature. b.The temperature of the first step in a PCR cycle. c.The temperature where half of the DNA molecules are denatured. d.Tm is the same as the annealing temperature. e.The Temperature at which all of the DNA molecules are dentaturedMost PCR reactions do not use the more expensive types of DNA polymerase, which have DNA proofreading. How might this be a problem in accurately copying specific DNA sequences of the target gene?The ingredients used in PCR will contain all of the following except: Group of answer choices A. primers B. ribosomes C. DNA polymerase D. nucleotide monomers
- Select all that apply: Which of these components must be added to a PCR reaction for it to produce a product?O DNA PrimersO Buffers- dNTPsO RNA PrimersO NTPSO PrimaseO Template DNAO Taq DNA PolymeraseThe PCR reaction contains deoxynucleotide triphosphates (dNTPs) in order to construct new DNA. There are four different dNTPs used in this reaction. Which answer below lists the four different dNTPs used in PCR? A) DNA polymerase, helicase, ligase, topoisomerase B) adenine, guanine, cytosine, thymine C) adenine, guanine,cytosine, uracil D) Alanine, guanine, cytosine, Theron oneThe image below represents the PCR results after electrophoresis on an agarose gel. One band is labelled as (i) and another is labelled as (ii). Which of the following descriptions below best describes the band labelled as (i)? a. Relaxed DNA b. Primers c. RNA d. Amplicon e. Digested DNA fragment f. None of the above