An object is 20µm in diameter. If viewed through a 10X ocular lens and a 40X objective lens, how large will the object appear (what are the magnified dimensions of the object)?
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- 1. In microscopy, what could be the possible reason why we cannot completely resolve the specimen under the Oil Immersion Objective (OIO) when, in fact, it was taught that the OIO has a resolving poewer? 2. When using the OIO, we can only focus on one group or parts at a time. (that is, the other parts are blurred) Whys is it so?1. What are the different hoods in the lab and why are they important? 2. How does the light compound microscope magnify an object? Outline the process.1. What is the wavelength used for the spectrophotometer? What is the principle of the spectrophotometer?
- 1)You can see something at 10x but not at 40x or 100x, what do you do? 2)Your microscope has light, the slide looks like it's in the right place, but you can't fully focus on the smear. Do you move up to the next magnification? Explain why?1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?4. How does the light compound microscope magnify an object? Outline the process5. Explain why an inverted image is seen under a compound microscope.6. What is the purpose of the oil when using oil immersion objective?
- 1. The resolving power of a microscope is a function ofa. the magnifying power of the lenses.b. the numerical aperture of the lenses.c. the wavelength of light.d. Both (a) and (b) are correct.e. Both (b) and (c) are correct.1. You're using a microscope with 10x magnification ocular lenses and a 25x magnification objective lens. What is the microscope's overall magnification at this setting? 2. Is it correct that immersion oil improves image magnification and resolution?1. What three things change as you increase magnification? 2. How do you properly use a microscope?
- 1. Name the part of the microscope that you use to control the cone of light coming through the condenser. 2. What is the total magnification of your specimen you are viewing using the 40X lens?3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm3. Explain the following terms and what are its corresponding requirements for Raman applications: a. Confocal microscope b. Numerical aperture c. Infinity correction d. F-number