Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter1: The Clinical Laboratory
Section1.10: The Microscope
Problem 18RQ
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  • Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes

the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases.

  • Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens
  • State the relationship between the working distance of an objective lens and its magnification power
  • “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it.
  • When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do?
  • What does this statement mean: “Your microscope is parfocal”?
  • What is the significance of condenser lens?
  • Multiple choice question:

The most useful adjustment for increasing image contrast in low power magnification is _______

  1. Closing down the diaphragm
  2. Closing one eye
  3. Opening up the diaphragm
  4. Placing a drop of oil on the slide
  5. Using a blue filer
  • How do you calculate the total magnification?
  • Explain why you must use oil immersion to conclude if the bacteria is Gram + or Gram -

 

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